Transcriptome-Based Analysis of the Oxidative Response of Thermotoga maritima to the O2 Stress.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Combinatorial chemistry & high throughput screening Pub Date : 2025-01-16 DOI:10.2174/0113862073339580241128075031

Raja Lakhal, Manaf AlMatar, Tahani Alkalaf, Osman Albarri

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Abstract

Background: Thermotoga maritima is an anaerobic hyperthermophilic eubacterium isolated from geothermally heated maritime surfaces. It can grow at temperatures up to 80 degrees Celsius.

Methods: A 2.3-L bioreactor was specifically designed to cultivate hyperthermophilic bacteria under carefully regulated pH, redox potential, temperature, and dissolved O2.

Results: Using this bioreactor, which was adjusted at 80°C and pH 7.0, it was found that Thermotoga maritima demonstrated continued growth even after being exposed to oxygen for an extended period. Transcription studies revealed that following prolonged oxygen exposure, the genes encoding ROS-scavenging systems, alkyl hydroperoxide reductase (ahp), thioredoxindependent thiol peroxidase (bcp 2), and, to a lesser extent, neelaredoxin (nlr), were upregulated/ overexpressed. When oxygen was available, the metabolism of glucose was diverted to make lactate rather than acetate.

Conclusion: Based on the O/R ratio of 1.0 in anaerobiosis and 1.67 in the presence of O2, we may conclude that Thermotoga maritima is capable of semi-oxidative metabolism.

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基于转录组的海洋热藻对O2胁迫的氧化反应分析。

背景：海洋热菌是一种从地热加热的海洋表面分离出来的厌氧嗜热真细菌。它可以在高达80摄氏度的温度下生长。方法：设计2.3 l生物反应器，在精心调节pH、氧化还原电位、温度和溶解氧条件下培养嗜热细菌。结果：采用该生物反应器，调节温度为80°C， pH为7.0，发现即使在长时间暴露于氧气中，Thermotoga marima也能持续生长。转录研究显示，在长时间的氧暴露后，编码ros清除系统的基因，烷基氢过氧化物还原酶（ahp），硫氧还不依赖的硫醇过氧化物酶（bcp 2），以及在较小程度上，neelaredoxin （nlr）上调/过表达。当氧气可用时，葡萄糖的代谢被转移为乳酸而不是醋酸盐。结论：无氧条件下O/R为1.0，有氧条件下O/R为1.67，表明海洋热藻具有半氧化代谢能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.