Resveratrol Anti-inflammatory Effect against Palmitate-induced Cytotoxicity in Raw 264.7 Macrophages.

IF 3.5 4区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Current medicinal chemistry Pub Date : 2025-01-01 DOI:10.2174/0109298673352457241210083325

Ekramy M Elmorsy, Ayat B Al-Ghafari, Huda A Al Doghaither

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Abstract

Background: Resveratrol (RES) is a phytochemical bioactive compound with suggested therapeutic benefits.

Objective: The current work aimed to evaluate the anti-inflammatory effect of RES against palmitate (PA) induced lipotoxicity in raw 264.7 macrophages cell line.

Methods: The cells viability was assessed by lactate dehydrogenase assay. Then the effects of RES and PA on nitric oxide (NO), triglyceride (TG) content, and cytokines release were studied. The effect of RES and PA on the treated cells bioenergetics and redox status was evaluated via different assays.

Results: The results showed that at doses of 10 and 20 μM, RES dramatically increased the vitality of PA-exposed macrophages with dramatic significant decrease in the release the proinflammatory cytokines TNF-α, HMGB-1, IL-1β, and IL-6 and their coding genes expression with marked improvement in the cells phagocytic capacity. In addition, RES dramatically lowered the levels of NO and TG in PA-stimulated macrophages. In addition, PA markedly decreased mitochondrial complexes I and III activities with decreased mitochondrial membrane potential and lowered ATP production with induction of oxidative stress. RES was shown to mitigate the effect of PA on macrophages bioenergetics and the oxidative damage and counteracted PA effect on genes linked to oxidative damage, such as Nrf2, Ho-1, NF-κB p65, SOD1, and SOD2.

Conclusion: RES could reduce PA-induced lipotoxicity in macrophages via enhancing their viability and counteracting the excess release of cytokines through alleviating PAinduced bioenergetic disruption and oxidative damage with a suggested positive impact of RES on obesity related illnesses caused by triggered cellular inflammation.

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白藜芦醇抗棕榈酸盐诱导的巨噬细胞毒性作用。

背景：白藜芦醇（Resveratrol， RES）是一种具有治疗作用的植物化学生物活性化合物。目的：研究RES对棕榈酸酯（PA）诱导的巨噬细胞264.7脂毒性的抗炎作用。方法：采用乳酸脱氢酶法测定细胞活力。然后研究RES和PA对一氧化氮（NO）、甘油三酯（TG）含量和细胞因子释放的影响。结果表明：在10 μ m和20μM剂量下，RES可显著提高PA暴露的巨噬细胞的活力，显著降低促炎因子TNF-α、MHGB-1、IL-1β和IL-6的释放及其编码基因的表达，显著提高细胞的吞噬能力。此外，RES显著降低pa刺激的巨噬细胞中NO和TG的水平。此外，PA显著降低线粒体复合体I和III的活性，降低线粒体膜电位，并通过氧化应激诱导降低ATP的产生。研究显示，RES可减轻PA对巨噬细胞生物能量学和氧化损伤的影响，并抵消PA对氧化损伤相关基因Nrf2、Ho-1、NF-κB p65、SOD1和SOD2的影响。结论：RES可通过增强巨噬细胞活力和抑制细胞因子的过度释放来减轻pa诱导的巨噬细胞脂毒性，从而减轻疼痛引起的生物能量破坏和氧化损伤，提示RES对由触发的细胞炎症引起的肥胖相关疾病具有积极作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current medicinal chemistry 医学-生化与分子生物学

CiteScore

8.60

自引率

2.40%

发文量

468

审稿时长

3 months

期刊介绍： Aims & Scope Current Medicinal Chemistry covers all the latest and outstanding developments in medicinal chemistry and rational drug design. Each issue contains a series of timely in-depth reviews and guest edited thematic issues written by leaders in the field covering a range of the current topics in medicinal chemistry. The journal also publishes reviews on recent patents. Current Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments.