Separation of Three Polar Compounds with Similar Polarities from Swertia mussotii by a Combination of Two Counter-Current Chromatography Modes.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of chromatographic science Pub Date : 2025-01-14 DOI:10.1093/chromsci/bmaf006

Hongmei Li, Tao Chen, Cheng Shen, Shuo Wang, Juyuan Luo, Zhibo Song, Yumei Ma, Aijing Li, Weihang Lu, Hailun Feng, Yulin Li

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Abstract

Separation of polar compounds especially with similar polarities is challenging. In the present study, three polar compounds with similar polarities, including gentiopicroside, sweroside and mangiferin, have been successfully separated from Swertia mussotii by a combination of two counter-current chromatography modes. With the selected solvent system of ethyl acetate/n-butanol/water (8/2/10, v/v), a continuous injection mode was firstly employed. As a result, 10.4 g of mangiferin with purity higher than 98% and 8 g of Peak I mainly composed of gentiopicroside and sweroside could be easily obtained from 24 g of the sample by counter-current chromatography through eight injections. Then, a recycling counter-current chromatography mode was introduced for the separation of gentiopicroside and sweroside. Through six cycles, 5.3 g of gentiopicroside and 0.8 g of sweroside with purity higher than 98% were obtained from 8 g of Peak I. The research provides a reference for the large-scale separation of polar compounds with similar polarities.

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两种逆流色谱法联合分离獐牙菜中极性相近的3种极性化合物。

极性化合物，特别是极性相似的极性化合物的分离是具有挑战性的。本研究采用两种逆流色谱方法，成功地从獐牙菜中分离出极性相似的三种极性化合物，包括龙胆苦苷（gentiopicroside）、苦苷（sweroside）和mangiferin。选择乙酸乙酯/正丁醇/水（8/2/10,v/v）溶剂体系，首次采用连续进样方式。结果表明，24 g样品经8次进样，通过反流色谱法可轻松得到纯度大于98%的芒果苷10.4 g和主要由龙胆苦苷和苦苷组成的I峰8 g。然后，采用循环逆流色谱法分离龙胆苦苷和苦苷。通过6个循环，从8 g峰i中分离得到纯度大于98%的龙心苦苷5.3 g和苦苷0.8 g，为极性相近的极性化合物的大规模分离提供了参考。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.