Identification and quantitative trait locus mapping of Tartary buckwheat pre-harvest sprouting

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2025-01-17 DOI:10.1002/jsfa.14117

Liwei Zhu, Yong Lv, Taoxiong Shi, Juan Huang, Qianqian Du, Guohong Tang, Genlou Sun, Odika Prince, Qingfu Chen

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Abstract

Background

Tartary buckwheat (Fagopyrum tartaricum) is particularly vulnerable to pre-harvest sprouting (PHS) due to its extended flowering and fruiting cycle, especially during periods of prolonged rainfall. This susceptibility has significant adverse effects on yield, quality and post-harvest processing. In this study, a recombinant inbred lines (RILs) population (XJ-RILs) was developed from a cross between the PHS-susceptible Tartary buckwheat variety ‘Xiaomiqiao’ (female parent) and the highly PHS-resistant variety ‘Jinqiaomai 2’ (male parent). Key traits, including germination percentage, germination energy, germination index, field PHS (PHS-F) and simulated PHS (PHS-S), were evaluated, and a quantitative trait locus (QTL) mapping analysis was performed.

Results

(i) PHS-S was strongly and significantly correlated with PHS-F. (ii) A total of 11 QTLs associated with seed germination and 14 QTLs related to PHS were identified. Notably, the major QTL cluster qPHS8-1 was consistently detected and mapped within the interval of 8.53–9.65 Mbp on chromosome Ft8. (iii) Genotyping of 221 XJ-RILs across eight chromosomes revealed five residual heterozygous lines carrying a heterozygous interval of qPHS8-1 cluster, with inbred line R56 being particularly suited for the fine mapping of qPHS8-1.

Conclusion

The PHS-S test, conducted on entire Tartary buckwheat spikes, is an effective and comprehensive method for assessing PHS resistance in this crop. QTL mapping identified qPHS8-1 as a major locus for PHS resistance, and inbred line R56 offers a promising resource for further fine mapping of this cluster. © 2025 Society of Chemical Industry.

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苦荞采前发芽鉴定及数量性状位点定位。

背景：苦荞（Fagopyrum tartaricum）特别容易受到收获前发芽（PHS）的影响，因为它的开花和结果周期延长，特别是在长时间降雨期间。这种敏感性对产量、品质和采后加工都有显著的不利影响。本研究利用对稻瘟病敏感的苦荞品种“小米翘”（母本）与稻瘟病抗性强的品种“金乔麦2号”（父本）杂交，构建了重组自交系（RILs）群体XJ-RILs。对发芽率、萌发能、萌发指数、田间小灵通（PHS- f）和模拟小灵通（PHS- s）等关键性状进行评价，并进行数量性状位点（QTL）定位分析。结果：(1)PHS-S与PHS-F呈显著强相关。（ii）共鉴定出11个与种子萌发相关的qtl和14个与小灵通相关的qtl。值得注意的是，主要QTL集群qPHS8-1在Ft8染色体8.53-9.65 Mbp的区间内被一致检测和定位。（iii）对8条染色体上221个xj - ril进行基因分型，发现5个残留杂合系携带qPHS8-1簇杂合区间，其中自交系R56特别适合qPHS8-1的精细定位。结论：对苦荞全穗进行PHS- s试验，是评价苦荞小灵通抗性的有效、综合的方法。QTL定位鉴定出qPHS8-1是小灵通抗性的主要位点，自交系R56为该群体的进一步精细定位提供了良好的资源。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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