Treatment effects of nitrogen and phosphorus addition on foliar traits in six northern hardwood tree species.

Abstract

Foliar traits can reflect fitness responses to environmental changes, such as changes in nutrient availability. Species may respond differently to these changes due to differences in traits and their plasticity. Traits and community composition together can influence forest nutrient cycling. We compared five traits-foliar N, foliar P, specific leaf area (SLA), leaf dry matter content (LDMC), and leaf carbon isotope ratio (δ¹³C)-in six northern hardwood tree species (Acer rubrum, Acer saccharum, Betula alleghaniensis, Betula papyrifera, Fagus grandifolia, and Prunus pensylvanica) in a nitrogen (N) and phosphorus (P) fertilization study across 10 mid- and late-successional forest stands in New Hampshire, USA. We also analyzed the response of tree growth to N and P addition. Nutrient addition shifted trait values towards the "acquisitive" side of the spectrum for all traits except δ¹³C, reflecting a tradeoff between water-use efficiency and nutrient-use efficiency. Treatment responses in relative basal area increment revealed that the Betula species were N-limited, but traits of all species responded to either or both N and P addition in ways that suggest N and P co-limitation. Two species displayed lower foliar P under N addition, and three species displayed lower foliar N under P addition, which also suggests co-limitation. These indications of co-limitation were reflected at the community level. Specific leaf area, LDMC, and δ¹³C differed with stand age within several species. Examining trait responses of tree species and communities to nutrient availability increases our understanding of biological mechanisms underlying the complex effects of nutrient availability on forests.