Comparison of Vegetable Waste Byproducts of Selected Cultivars of Foeniculum vulgare Mill. by an Integrated LC-(HR)MS and ¹H-NMR-Based Metabolomics Approach.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Phytochemical Analysis Pub Date : 2025-01-21 DOI:10.1002/pca.3488

Maria Assunta Crescenzi, Antonietta Cerulli, Milena Masullo, Paola Montoro, Sonia Piacente

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Abstract

Introduction: The metabolome of plants is influenced by various factors, especially environmental, as the season in which they are grown. So, distinct varieties of the identical plant might show an increase or decrease in metabolites. The diversity of content of primary and secondary metabolites can also determine the variation in their biological properties. Due to the current occurrence of various fennel varieties, the crop can now be grown for the entire year.

Objective: This work used an integrated approach of LC/MS and NMR analysis to characterize the metabolome of fennel waste of different varieties by multivariate statistical analysis.

Methods: The extracts were investigated by NMR and LC/MS analysis to focus attention on the primary and secondary metabolites. Both LC-HRMS and NMR data were analyzed by principal component analysis (PCA).

Results: The ¹H-NMR analysis led to the identification of 15 primary metabolites, such as amino acids, carbohydrates, and organic acid derivatives. The secondary metabolites identified by LC/MS analysis mainly belong to the phenolic, lipid, and fatty acid compounds classes.

Conclusion: This integrated approach guarantees a precise and complete overview of the variations in the metabolic expression of the fennel varieties grown in different seasons.

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小茴香不同栽培品种蔬菜废弃物的比较。基于LC-(HR)MS和1h - nmr的代谢组学方法。

植物的代谢组受多种因素的影响，尤其是生长季节等环境因素的影响。因此，同一种植物的不同品种可能会表现出代谢物的增加或减少。初级代谢物和次级代谢物含量的多样性也决定了其生物学特性的差异。由于现在出现了各种茴香品种，这种作物现在可以全年种植。目的：采用LC/MS和NMR相结合的方法对不同品种茴香废弃物代谢组进行多元统计分析。方法：采用核磁共振（NMR）和质谱（LC/MS）对提取物进行一级和二级代谢产物分析。采用主成分分析（PCA）对LC-HRMS和NMR数据进行分析。结果：1H-NMR分析鉴定出15种主要代谢物，如氨基酸、碳水化合物和有机酸衍生物。经LC/MS分析鉴定的次生代谢产物主要为酚类、脂类和脂肪酸类化合物。结论：这种综合方法保证了对不同季节生长的茴香品种代谢表达变化的精确和完整的概述。

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.