Evaluation of the Effect of Exosomes From Adipose Derived Stem Cells on Changes in GSH/ROS Levels During Skin Photoaging.

IF 2.5 4区医学 Q2 DERMATOLOGY Photodermatology, photoimmunology & photomedicine Pub Date : 2025-01-01 DOI:10.1111/phpp.70009

Yiping Wang, Xu Shen, Shenghua Song, Nian Chen, Yihao Wang, Wanxing Liao, Chiyu Jia, Li Zeng

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Abstract

Objective: Exosomes (Exos) from adipose derived stem cells (ADSCs) can delay skin photoaging, but their effects on reactive oxygen species (ROS) remains unclear. This study aimed to investigate the relationship between adipose derived stem cell exosomes (ADSCs-Exos) in anti-photoaging of skin and glutathione (GSH)/ ROS expression in human fibroblasts.

Methods: A skin photoaging model was established by irradiating human fibroblasts with ultraviolet B (UVB) light in vitro. Next, exosomes from ADSCs were isolated for treating the photoaged fibroblasts. Afterwards, the alterations in photoaged fibroblasts were analyzed by a series of assays including senescence-associated β-galactosidase (SA-β-Gal) staining, p16 expression, ROS staining, and GSH content.

Results: After a human fibroblast photoaging model was subjected to ADSCs-Exos treatment, we found that the high concentration exosome group had the highest GSH content. Cellular staining showed that levels of SA-β-Gal, p16, and ROS of the high concentration-treated group were lower than other groups.

Conclusions: ADSCs-Exos can protect skin fibroblasts from photoaging via increasing the ratio of GSH/ROS.

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评估脂肪干细胞外泌体对皮肤光老化过程中GSH/ROS水平变化的影响。

目的：来自脂肪干细胞（ADSCs）的外泌体（Exos）可以延缓皮肤光老化，但其对活性氧（ROS）的影响尚不清楚。本研究旨在探讨抗皮肤光老化的脂肪源性干细胞外泌体（ADSCs-Exos）与人成纤维细胞谷胱甘肽(GSH)/ ROS表达的关系。方法：用紫外线B （UVB）光体外照射人成纤维细胞，建立皮肤光老化模型。接下来，从ADSCs中分离外泌体用于处理光老化的成纤维细胞。随后，通过衰老相关β-半乳糖苷酶（SA-β-Gal）染色、p16表达、ROS染色和GSH含量等一系列检测分析光老化成纤维细胞的变化。结果：人成纤维细胞光老化模型经ADSCs-Exos处理后，我们发现高浓度外泌体组GSH含量最高。细胞染色显示，高浓度处理组SA-β-Gal、p16、ROS水平低于其他各组。结论：ADSCs-Exos可通过提高GSH/ROS的比值来保护皮肤成纤维细胞的光老化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photodermatology, photoimmunology & photomedicine 医学-皮肤病学

CiteScore

4.40

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal is a forum for new information about the direct and distant effects of electromagnetic radiation (ultraviolet, visible and infrared) mediated through skin. The divisions of the editorial board reflect areas of specific interest: aging, carcinogenesis, immunology, instrumentation and optics, lasers, photodynamic therapy, photosensitivity, pigmentation and therapy. Photodermatology, Photoimmunology & Photomedicine includes original articles, reviews, communications and editorials. Original articles may include the investigation of experimental or pathological processes in humans or animals in vivo or the investigation of radiation effects in cells or tissues in vitro. Methodology need have no limitation; rather, it should be appropriate to the question addressed.