A cross-sectional study on the prevalence of multidrug-resistant clinical isolates of Proteus species in Northern Iran.

IF 1.4 4区医学 Q4 INFECTIOUS DISEASES Journal of Infection in Developing Countries Pub Date : 2024-12-31 DOI:10.3855/jidc.17637

Mahnaz Shafaei Fallah, Hadi Razavi Nikoo, Aylar Jamali, Alireza Mohebbi, Ezzat Allah Ghaemi

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Abstract

Introduction: Multidrug-resistant (MDR) bacteria like Proteus species have led to more prolonged hospitalizations, fewer care choices, higher treatment costs, and even death. The present study aims to evaluate the prevalence of MDR Proteus species in clinical samples and to suggest the best therapeutic options for the MDR Proteus species.

Methodology: Clinical samples were collected randomly from five hospitals in Golestan Province, Iran, from February 2017 to July 2019. Disk diffusion on Mueller-Hinton agar plates were used to perform antibiotic susceptibility testing (ASTs). By using a double-disc synergy test (DDST), isolates resistant to one of the third-generation cephalosporins were examined for phenotypic extended-spectrum β-lactamase (ESBL) development. A combined double disk synergy test (CDDST) was used to identify MBL-producing isolates.

Results: 61 Proteus isolates, including P. Mirabilis 44/61 (77.04%), P. vulgaris 7/61 (11.47%), P. hauseri 5/61 (8.19%), and P. penneri 2/61 (3.27%) were collected. Most of the isolates were obtained from urine samples. P. hauseri isolates were more frequent in females. Resistance to tetracycline and nitrofurantoin antibiotics was observed in most Proteus isolates. P. penneri isolates were all resistant to antibiotics. ESBL production was observed in five ceftazidime-resistant isolates (p < 0.05).

Conclusions: Cefepime and imipenem were found to have the lowest occurrence of antibiotic resistance among Proteus species, confirming that cefepime and imipenem can be used to treat Proteus infections.

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伊朗北部变形杆菌多种耐药临床分离株流行情况的横断面研究。

简介：像变形杆菌这样的耐多药（MDR）细菌导致更长的住院时间、更少的护理选择、更高的治疗费用，甚至死亡。本研究旨在评估耐多药变形菌在临床样本中的流行情况，并提出耐多药变形菌的最佳治疗方案。方法：2017年2月至2019年7月在伊朗戈列斯坦省5家医院随机抽取临床样本。采用Mueller-Hinton琼脂平板纸片扩散法进行抗生素敏感性试验（ast）。采用双盘协同试验（DDST）检测了对第三代头孢菌素耐药的分离株的表型扩展谱β-内酰胺酶（ESBL）发育情况。采用联合双盘协同试验（CDDST）鉴定产生mbl的菌株。结果：共收集到变形杆菌61株，其中奇异P. 44/61（77.04%）、寻常P. 7/61（11.47%）、hauseri P. 5/61（8.19%）、penneri P. 2/61（3.27%）。大多数分离株来自尿液样本。hauseri分离株在女性中更为常见。大多数变形杆菌分离株对四环素和呋喃妥因类抗生素耐药。penneri菌株均对抗生素耐药。5株头孢他啶耐药菌株产生ESBL （p < 0.05）。结论：头孢吡肟和亚胺培南在变形杆菌中耐药发生率最低，证实头孢吡肟和亚胺培南可用于变形杆菌感染的治疗。

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来源期刊

Journal of Infection in Developing Countries INFECTIOUS DISEASES-

CiteScore

3.70

自引率

5.30%

发文量

239

审稿时长

4-8 weeks

期刊介绍： The Journal of Infection in Developing Countries (JIDC) is an international journal, intended for the publication of scientific articles from Developing Countries by scientists from Developing Countries. JIDC is an independent, on-line publication with an international editorial board. JIDC is open access with no cost to view or download articles and reasonable cost for publication of research artcles, making JIDC easily availiable to scientists from resource restricted regions.