Investigation Of The Effects Of Hyperbaric Oxygen Therapy On Hepatotoxicity Induced By Leflunomide in Rats.

Abstract

Background: Hyperbaric Oxygen Therapy (HBO₂) is a treatment modality that exposes patients to 100% oxygen at higher atmospheric pressures. Recently, HBO₂ has emerged as a potential therapeutic option for various liver diseases, offering advantages such as improved tissue oxygenation, anti-inflammatory effects, enhanced wound healing, and potential hepatoprotective properties. Understanding the benefits of HBO₂ in liver diseases can pave the way for novel therapeutic strategies and improved patient outcomes. This study aimed to investigate the hepatoprotective effect of HBO₂ in arthritic rats treated with leflunomide (LEF) through anti-inflammatory and antioxidant pathways.

Material and methods: 24 male Sprague-Dawley rats were divided into three groups (8 animals in each group (n = 8)). 1^st group was the control group, which received no treatment. 2^nd group was RA + LEF 5 mg/kg, 3^rd group was RA + LEF 5 mg/kg + HBO₂. Rheumatoid arthritis was induced using Complete Freund's Adjuvant (CFA). The treatment was initiated on the 10^th day following induction and lasted for a total of 18 days. The impact on disease progression was assessed through histological changes, which were evaluated using hematoxylin-eosin staining, while the Anti-TNF-α antibody levels were determined.

Results: TCompared with the RL group, the RLH group significantly decreases necrotic cells, Lymphocyte- rich mononuclear cells, and active anti-TNF-α .

Conclusion: HBO₂ showed a beneficial effect and decreased hepatotoxicity on Leflunomide-induced liver injury.