{"title":"Weak or partial D: Importance of molecular characterization of D variants","authors":"Akshaya Tomar , Rati Devendra , Disha Parchure , Swati Kulkarni","doi":"10.1016/j.transci.2025.104069","DOIUrl":null,"url":null,"abstract":"<div><div>This case report presents first case of <em>RHD*weak D type 9</em> in a 38-year-old Indian patient with severe osteoarthritis of the left hip joint scheduled for total hip replacement surgery. During routine blood grouping, an unexpected weak reaction with anti-D was observed. Serological characterization using an extended partial D typing kit characterized the variant as DV. The patient was successfully transfused with a O RhD negative compatible unit as per transfusion guidelines after surgery, without any observed transfusion reactions. The blood sample was sent to the ICMR-National Institute of Immunohematology, Mumbai, where molecular characterization revealed the presence of an <em>RHD* 01 W.9</em> allele, as the underlying cause of weak expression of the RhD antigen. Serology can at best lead to the detection of the presence of a variant but characterization requires the help of molecular techniques.</div></div>","PeriodicalId":49422,"journal":{"name":"Transfusion and Apheresis Science","volume":"64 2","pages":"Article 104069"},"PeriodicalIF":1.4000,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transfusion and Apheresis Science","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1473050225000035","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"HEMATOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This case report presents first case of RHD*weak D type 9 in a 38-year-old Indian patient with severe osteoarthritis of the left hip joint scheduled for total hip replacement surgery. During routine blood grouping, an unexpected weak reaction with anti-D was observed. Serological characterization using an extended partial D typing kit characterized the variant as DV. The patient was successfully transfused with a O RhD negative compatible unit as per transfusion guidelines after surgery, without any observed transfusion reactions. The blood sample was sent to the ICMR-National Institute of Immunohematology, Mumbai, where molecular characterization revealed the presence of an RHD* 01 W.9 allele, as the underlying cause of weak expression of the RhD antigen. Serology can at best lead to the detection of the presence of a variant but characterization requires the help of molecular techniques.
期刊介绍：
Transfusion and Apheresis Science brings comprehensive and up-to-date information to physicians and health care professionals involved in the rapidly changing fields of transfusion medicine, hemostasis and apheresis. The journal presents original articles relating to scientific and clinical studies in the areas of immunohematology, transfusion practice, bleeding and thrombotic disorders and both therapeutic and donor apheresis including hematopoietic stem cells. Topics covered include the collection and processing of blood, compatibility testing and guidelines for the use of blood products, as well as screening for and transmission of blood-borne diseases. All areas of apheresis - therapeutic and collection - are also addressed. We would like to specifically encourage allied health professionals in this area to submit manuscripts that relate to improved patient and donor care, technical aspects and educational issues.
Transfusion and Apheresis Science features a "Theme" section which includes, in each issue, a group of papers designed to review a specific topic of current importance in transfusion and hemostasis for the discussion of topical issues specific to apheresis and focuses on the operators'' viewpoint. Another section is "What''s Happening" which provides informal reporting of activities in the field. In addition, brief case reports and Letters to the Editor, as well as reviews of meetings and events of general interest, and a listing of recent patents make the journal a complete source of information for practitioners of transfusion, hemostasis and apheresis science. Immediate dissemination of important information is ensured by the commitment of Transfusion and Apheresis Science to rapid publication of both symposia and submitted papers.
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