[Elucidation of Coloring Molecule Using a Simple Identification Method with a Color Reaction for Omphalotus japonicus].

IF 0.2 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI:10.3358/shokueishi.65.137
Hideyuki Shinohara, Ryuma Okawara, Ryota Akatsuka, Masahiro Sato, Yuka Nagaoka
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Abstract

For Omphalotus japonicus, the coloring molecule was found and characterized using a simple method of identification with a color reaction. The compound that chang color under basic conditions was isolated from a methanolic extract of O. japonicus by liquid-liquid extraction. That compound was identified as thelephoric acid by various spectrum analyses. Color reaction by a beam reagent (5 w/v% potassium hydroxide ethanolic solution) and UV-Vis absorption of an ethanolic solution of thelephoric acid coincided with those of an ethanolic extract of O. japonicus. We conducted LC-MS/MS analyses of all four mushroom species (O. japonicus, Sarcomyxa serotina, Pleurotus ostreatus, and Lentinula edodes). Results demonstrated that thelephoric acid was detected only in O. japonicus. These findings indicate thelephoric acid as a coloring molecule for the identification method described above. This method is useful for distinguishing O. japonicus among other edible mushrooms having similar appearance.

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用一种简单的显色反应方法鉴定日本红花的显色分子。
用显色反应的简单鉴别方法,对日本参的显色分子进行了鉴定。采用液-液萃取法从黄参甲醇提取物中分离出碱性条件下变色的化合物。该化合物经各种光谱分析鉴定为象皮酸。用光束试剂(5w /v%的氢氧化钾乙醇溶液)进行显色反应,用乙醇溶液对象皮酸的紫外-可见吸收与用乙醇提取物对象皮酸的紫外-可见吸收一致。采用LC-MS/MS对4种蘑菇(O. japonicus, Sarcomyxa servtina, Pleurotus ostreatus, Lentinula edodes)进行分析。结果表明,象皮酸仅在日本参中检出。这些发现表明象皮酸可以作为上述鉴定方法的着色分子。该方法可用于在其他外观相似的食用菌中进行鉴别。
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来源期刊
Food Hygiene and Safety Science
Food Hygiene and Safety Science Medicine-Public Health, Environmental and Occupational Health
CiteScore
0.70
自引率
0.00%
发文量
28
审稿时长
18-36 weeks
期刊介绍: Information not localized
期刊最新文献
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