Evaluating CK20 and MCPyV Antibody Clones in Diagnosing Merkel Cell Carcinoma.

IF 11.3 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Endocrine Pathology Pub Date : 2025-01-22 DOI:10.1007/s12022-024-09845-w
Begum Yeni Erdem, Can Baykal, Yasemin Ozluk, Melin A Ahmed, Erol Kozanoglu, Pinar Saip, Nesimi Buyukbabani, Sule Ozturk Sari
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Abstract

Merkel cell carcinoma (MCC) is diagnosed through histopathological and immunohistochemical examination of biopsies from skin or other organs. Its distinguishing features include perinuclear dot-like staining with Cytokeratin 20 (CK20) and detection of Merkel cell polyomavirus (MCPyV) using various methods. However, CK20 and MCPyV negative MCC cases have been reported at varying rates. In this single center cross-sectional study, we aimed to determine which clones are more effective in diagnosing MCC by comparing the performance of CK20 antibody clones Ks20.8 and SP33, as well as MCPyV antibody clones Ab3 and CM2B4. Fifty-four patients diagnosed with MCC were included. Among these, 42 cases were primary cutaneous, and 12 cases were nodal MCC. Fifty-two (96.3%) cases were positive with both CK20 clones, while two cases were negative. Clone SP33 stained areas of necrosis, whereas Ks20.8 showed no aberrant staining. MCPyV was detected in 44 cases (81.5%) using clone Ab3 and 39 cases (72.2%) using clone CM2B4. Staining with MCPyV clone Ab3 was diffuse and strong in most cases, while approximately 30% of CM2B4-positive cases exhibited low percentages and/or weak staining, complicating the evaluation. The two CK20-negative cases were also negative with both MCPyV clones. Our data demonstrated that CK20 clone Ks20.8 may be preferred for MCC diagnosis due to its consistent performance and lack of aberrant staining. Similarly, MCPyV clone Ab3 appears superior to CM2B4 for identifying MCPyV-positive cases.

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CK20和MCPyV抗体克隆在默克尔细胞癌诊断中的价值
默克尔细胞癌(MCC)是通过皮肤或其他器官活检的组织病理学和免疫组织化学检查来诊断的。其显著特征包括核周细胞角蛋白20 (CK20)斑点样染色和使用各种方法检测默克尔细胞多瘤病毒(MCPyV)。然而,CK20和MCPyV阴性MCC病例的报道率不同。在这项单中心横断面研究中,我们旨在通过比较CK20抗体克隆Ks20.8和SP33以及MCPyV抗体克隆Ab3和CM2B4的表现来确定哪些克隆对MCC的诊断更有效。54名诊断为MCC的患者被纳入研究。其中原发皮肤MCC 42例,结性MCC 12例。52例(96.3%)CK20克隆均阳性,2例阴性。克隆SP33染色坏死区域,而Ks20.8无异常染色。克隆Ab3检出MCPyV 44例(81.5%),克隆CM2B4检出39例(72.2%)。MCPyV克隆Ab3染色在大多数病例中呈弥漫性和强染色,而大约30%的cm2b4阳性病例表现出低百分比和/或弱染色,使评估复杂化。两例ck20阴性病例的MCPyV克隆也均为阴性。我们的数据表明,CK20克隆Ks20.8可能是MCC诊断的首选,因为它的表现一致,没有异常染色。同样,MCPyV克隆Ab3在识别MCPyV阳性病例方面优于CM2B4。
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来源期刊
Endocrine Pathology
Endocrine Pathology 医学-病理学
CiteScore
12.30
自引率
20.50%
发文量
41
审稿时长
>12 weeks
期刊介绍: Endocrine Pathology publishes original articles on clinical and basic aspects of endocrine disorders. Work with animals or in vitro techniques is acceptable if it is relevant to human normal or abnormal endocrinology. Manuscripts will be considered for publication in the form of original articles, case reports, clinical case presentations, reviews, and descriptions of techniques. Submission of a paper implies that it reports unpublished work, except in abstract form, and is not being submitted simultaneously to another publication. Accepted manuscripts become the sole property of Endocrine Pathology and may not be published elsewhere without written consent from the publisher. All articles are subject to review by experienced referees. The Editors and Editorial Board judge manuscripts suitable for publication, and decisions by the Editors are final.
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