Impact of triclocarban exposure on development and gene expression in the wolf spider, Pardosa pseudoannulata (Araneae: Lycosidae)

Abstract

Triclocarban (TCC), an emerging organic contaminant, poses a potential threat to water resources and ecosystems. The wolf spider Pardosa pseudoannulata (Araneae: Lycosidae) is a dominant predator typically inhabiting rice fields or wet habitats near water sources. However, little is known about the effects of TCC on the wolf spiders. In the present study, using environmentally relevant concentrations, we systematically investigated the toxicity of TCC on the early life stages of P. pseudoannulata at both physiological and gene expression levels. Our results indicated that TCC exposure did not significantly affect the survival and development of spiderlings. Transcriptome analysis revealed that TCC stress significantly impacted drug metabolism, cell cycle and signal transduction pathways in spiderlings. Subsequently, qRT-PCR was used to verify the expression levels of genes associated with drug metabolism and cell process, and the results were consistent with the transcriptome. These findings enhance our understanding of the toxic mechanism and ecological risk of TCC on arthropods.