Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1^® Advanced XL System.

IF 2 Q3 BIOCHEMICAL RESEARCH METHODS Methods and Protocols Pub Date : 2025-01-02 DOI:10.3390/mps8010002

Scarlet Neilson, Leah Nangeroni, Mirna Ghemrawi

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Abstract

This differential extraction protocol details the steps for isolating DNA from sample pads used in lateral flow immunochromatographic (LFI) tests, particularly for cases involving mixed biological samples such as semen and menstrual blood, or other evidence related to sexual assault. This procedure utilizes a differential extraction technique applied to sample pads from immunochromatographic tests, where the sample pads serve as the substrate. The method involves two sequential lysis steps to effectively separate non-sperm and sperm fractions, enabling the targeted isolation of distinct cell types for downstream DNA analysis. The efficiency of this procedure is demonstrated by the results within this paper, which highlights the successful recovery of both male autosomal and Y-STR profiles, even in mixed samples with a high female presence. Overall, this protocol demonstrates the effective recovery of DNA from sample pads, which is beneficial for forensic practitioners dealing with limited sample quantities, underscoring the value of using these pads in forensic analysis.

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通过EZ1®高级XL系统从横向流动免疫层析测试中提取差异DNA。

本鉴别提取方案详细说明了从侧流免疫层析（LFI）测试中使用的样本垫中分离DNA的步骤，特别是涉及混合生物样本（如精液和经血）或与性侵犯有关的其他证据的情况。本程序利用应用于免疫层析测试样品垫的差分提取技术，其中样品垫作为底物。该方法包括两个连续的裂解步骤，以有效地分离非精子和精子部分，从而能够有针对性地分离不同的细胞类型，用于下游DNA分析。本文的结果证明了这一程序的效率，该结果强调了男性常染色体和Y-STR谱的成功恢复，即使在混合样本中也有很高的女性存在。总的来说，该协议证明了有效地从样本垫中恢复DNA，这对处理有限样本数量的法医从业者是有益的，强调了在法医分析中使用这些垫的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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