Rapid on-site detection of imazaquin residue in corn and soybeans using an immunochromatographic assay†

IF 6.1 3区医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Journal of Materials Chemistry B Pub Date : 2025-01-17 DOI:10.1039/D4TB02347A

Xuyang Ma, Lingling Guo, Liqiang Liu, Hua Kuang, Chuanlai Xu and Aihua Qu

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Abstract

Imazaquin (IMQ) is an imidazolinone group herbicide widely used for weed control around the world. Due to excessive use during crop production, IMQ can accumulate in corn and soybeans, positing a potential threat to human health. In this study, a hapten that had high specificity and sensitivity was designed using computer-simulated technology. A monoclonal antibody (mAb) was synthesized with a half inhibitory concentration of 0.98 ng mL⁻¹. The mAb was incorporated into an immunochromatographic assay (ICA) for the detection of IMQ in corn and soybeans. The visual limit of detection was 10 μg kg⁻¹ in corn with a linear range of 3.85–208.26 μg kg⁻¹ and 5 μg kg⁻¹ in soybeans with a linear range of 3.78–106.71 μg kg⁻¹. Additionally, the ICA strips had excellent recovery rates, and the results were confirmed by liquid chromatography tandem mass spectrometry. Our study developed a method for the rapid on-site detection of IMQ residues in corn and soybeans.

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免疫层析法快速现场检测玉米和大豆中吡虫啉残留。

Imazaquin （IMQ）是一种咪唑啉酮类除草剂，在世界范围内广泛应用于杂草防治。由于作物生产过程中过量使用，玉米和大豆中会积累IMQ，对人体健康构成潜在威胁。本研究利用计算机模拟技术设计了具有高特异性和敏感性的半抗原。合成单克隆抗体（mAb），半抑制浓度为0.98 ng mL-1。该单抗被纳入玉米和大豆中IMQ的免疫层析检测（ICA）。玉米的视觉检出限为10 μ kg-1，线性范围为3.85 ~ 208.26 μ kg-1；大豆的视觉检出限为5 μ kg-1，线性范围为3.78 ~ 106.71 μ kg-1。此外，ICA条带具有良好的回收率，并通过液相色谱串联质谱法证实了这一结果。本研究建立了玉米和大豆中IMQ残留的快速现场检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Chemistry B MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

11.50

自引率

4.30%

发文量

866

期刊介绍： Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive： Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices