Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy

IF 23.6 1区医学 Q1 ENGINEERING, BIOMEDICAL Bioactive Materials Pub Date : 2025-04-01 Epub Date: 2025-01-03 DOI:10.1016/j.bioactmat.2024.12.024

Xiang Lin , Anne M. Filppula , Yuanjin Zhao , Luoran Shang , Hongbo Zhang

{"title":"Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy","authors":"Xiang Lin , Anne M. Filppula , Yuanjin Zhao , Luoran Shang , Hongbo Zhang","doi":"10.1016/j.bioactmat.2024.12.024","DOIUrl":null,"url":null,"abstract":"<div><div>Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions. Stem cells have gained attention in anti-photoaging, while controlling the tissue mechanical microenvironment of cell delivery sites is crucial for regulating cell fate and achieving optimal therapeutic performances. Here, we introduce a mechanically regulated human recombinant collagen (RHC) microcarrier generated through microfluidics, which is capable of modulating stem cell differentiation to treat photoaged skin. By controlling the cross-linking parameters, the mechanical properties of microcarriers could precisely tuned to optimize the stem cell differentiation. The microcarriers are surface functionalized with fibronectin (Fn)-platelet derived growth factor-BB (PDGF-BB) to facilitate adipose derived mesenchymal stem cells (Ad-MSCs) loading. In <em>in vivo</em> experiments, subcutaneous injection of stem cell loaded RHC microcarriers significantly reduced skin wrinkles after ultraviolet-injury, effectively promoted collagen synthesis, and increased vascular density. These encouraging results indicate that the present mechanically regulated microcarriers have great potential to deliver stem cells and regulate their differentiation for anti-photoaging treatments.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 448-456"},"PeriodicalIF":23.6000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754972/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioactive Materials","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452199X24005619","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/3 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions. Stem cells have gained attention in anti-photoaging, while controlling the tissue mechanical microenvironment of cell delivery sites is crucial for regulating cell fate and achieving optimal therapeutic performances. Here, we introduce a mechanically regulated human recombinant collagen (RHC) microcarrier generated through microfluidics, which is capable of modulating stem cell differentiation to treat photoaged skin. By controlling the cross-linking parameters, the mechanical properties of microcarriers could precisely tuned to optimize the stem cell differentiation. The microcarriers are surface functionalized with fibronectin (Fn)-platelet derived growth factor-BB (PDGF-BB) to facilitate adipose derived mesenchymal stem cells (Ad-MSCs) loading. In in vivo experiments, subcutaneous injection of stem cell loaded RHC microcarriers significantly reduced skin wrinkles after ultraviolet-injury, effectively promoted collagen synthesis, and increased vascular density. These encouraging results indicate that the present mechanically regulated microcarriers have great potential to deliver stem cells and regulate their differentiation for anti-photoaging treatments.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

机械调节微载体与干细胞负载皮肤光老化治疗。

长期暴露在紫外线辐射下会损害皮肤结构的完整性，导致正常生理功能的破坏。干细胞在抗光老化方面受到关注，而控制细胞传递位点的组织机械微环境对于调节细胞命运和获得最佳治疗性能至关重要。在这里，我们介绍了一种通过微流体产生的机械调节的人重组胶原蛋白（RHC）微载体，它能够调节干细胞分化来治疗光老化皮肤。通过控制交联参数，可以精确调节微载体的力学性能，优化干细胞的分化。微载体被纤连蛋白(Fn)-血小板衍生生长因子- bb （PDGF-BB）表面功能化，以促进脂肪源性间充质干细胞（Ad-MSCs）的装载。在体内实验中，皮下注射装载RHC微载体的干细胞可显著减少紫外线损伤后的皮肤皱纹，有效促进胶原合成，增加血管密度。这些令人鼓舞的结果表明，目前机械调节的微载体在抗光老化治疗中具有巨大的潜力来运送干细胞并调节其分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.