Josef P Magyar, Roxana Simon, Monika Petus, Krisztina Rigó
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引用次数: 0
Abstract
One of the main endpoints for the evaluation of Developmental and Reproductive Toxicology (DART) studies is the determination of potential effects of a test substance on the skeleton during foetal development. In the course of a DART study according to the OECD 414 guideline, 400 to 500 gestational day 20-old (GD20), alizarin red and alcian blue-stained (ARAB) rat foetuses have to be assessed by a teratology expert, which is a time consuming and sub-optimally documented process. We have developed a method which allows for a standardised, comprehensive, quick and easy to perform, head-to-toe digital documentation of ARAB-stained GD20 rat foetuses. The method consists of the acquiring a video-stream or a time lapse-recording of foetuses, which for this purpose are axially rotated in a custom-made device. The obtained digital material is suitable for the complete visual inspection of stained foetal specimen from all sides in great detail, and thus can replace the conventional inspection under a binocular microscope, and might render the archiving of such specimen unnecessary.
期刊介绍：
Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine.
All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.
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