In Vitro Biochemical Control of Taenia solium and Taenia saginata Eggs

IF 1.2 3区农林科学 Q4 PARASITOLOGY Acta Parasitologica Pub Date : 2025-01-24 DOI:10.1007/s11686-024-00950-8

Debora Castro de Souza, Lisseth Bibiana Figueroa Puentes, Adriane Toledo da Silva, Dyesse Pollyane Ferreira, Carolina Magri Ferraz, Fabio Ribeiro Braga, Filippe Elias de Freitas Soares

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Abstract

Purpouse

The present study evaluated in vitro the action of the plant protease papain (EC 3.4.22.2) on intact eggs of Taenia solium and Taenia saginata.

Methods

Two experimental groups were composed for each of the gastrointestinal helminths: control group (G1) and treated group (G2). In each replicate corresponding to the control group, 100 µL of the suspension containing approximately 100 intact eggs (T. solium) or 50 eggs (T. saginata) and 100 µL of distilled water were added, while for the treated group 100 µL of the egg suspension and 100 µL of the papain solution at a concentration of 15% (m/v) were added. The experiment was conducted in four replicates. The groups were incubated in BOD at 28 ± 1 ºC in the dark for a period of 24 h.

Results

The results demonstrated that there was a significant reduction (p < 0.01) of intact eggs, with average reduction percentages for T. saginata and T. solium of 74.36% and 64.29%, respectively.

Conclusions

This study is the first report on the in vitro control of helminth eggs (T. saginata and T. solium) using pure papain.

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.