Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels.

IF 3.9 3区医学 Q1 ENGINEERING, MULTIDISCIPLINARY Biomimetics Pub Date : 2025-01-10 DOI:10.3390/biomimetics10010040

Nathan Wood, Esther I Doria, Taieba Tuba Rahman, Wanhe Li, Zhijian Pei, Hongmin Qin

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Abstract

In this preliminary study, the long-term effects of calcium chloride crosslinking concentration on viability of 16HBE14o- human bronchial epithelial cells embedded in alginate-extracellular matrix (ECM) or alginate-methylcellulose-ECM hydrogels have been investigated. There is currently a limited understanding regarding the effects of crosslinking solution concentration on lung epithelial cells embedded in hydrogel. Furthermore, the effects of calcium chloride concentration in crosslinking solutions on other cell types have not been reported regarding whether the addition of viscosity and stiffness tuning agents such as methylcellulose will alter the responses of cells to changes in calcium chloride concentration in crosslinking solutions. While there were no significant effects of calcium chloride concentration on cell viability in alginate-ECM hydrogels, there is a decrease in cell viability in alginate-methylcellulose-ECM hydrogels crosslinked with 300 mM calcium chloride crosslinking solution. These findings have implications in the maintenance of 16HBE14o- 3D cultures with respect to the gelation of alginate with high concentrations of ionic crosslinking solution.

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氯化钙交联溶液浓度对海藻酸盐基水凝胶包埋16HBE14o-人支气管细胞长期细胞活力的影响。

在本初步研究中，研究了氯化钙交联浓度对海藻酸盐-细胞外基质（ECM）或海藻酸盐-甲基纤维素-ECM水凝胶包埋的16HBE14o-人支气管上皮细胞活力的长期影响。目前对交联溶液浓度对水凝胶包埋肺上皮细胞的影响了解有限。此外，交联溶液中氯化钙浓度对其他细胞类型的影响尚未被报道，关于添加粘度和刚度调节剂（如甲基纤维素）是否会改变细胞对交联溶液中氯化钙浓度变化的反应。虽然氯化钙浓度对海藻酸盐- ecm水凝胶的细胞活力没有显著影响，但用300 mM氯化钙交联溶液交联的海藻酸盐-甲基纤维素- ecm水凝胶的细胞活力有所下降。这些发现对维持16HBE14o- 3D培养物与高浓度离子交联溶液的海藻酸盐凝胶化具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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