Characterisation of Proteus mirabilis isolates from the poultry production chain in Zhejiang Province, China: antimicrobial resistance, virulence factors and genotypic profiling.

Abstract

1. This study investigated antimicrobial resistance, virulence factors and genotypic profiling among Proteus mirabilis isolated from three sources (poultry farms, slaughterhouses and retail markets) in the poultry production chain in Zhejiang Province, China, to assess its potential risk to public health.2. A total of 112 P. mirabilis strains were isolated from 409 samples, including 35 from poultry farms, 35 from slaughterhouses and 42 from retail markets. Antimicrobial susceptibility was tested using 18 antimicrobials in 9 categories, in which 110 (98.2%) strains were considered multidrug-resistant (MDR). These strains carried numerous antimicrobial resistance genes, with the sulphonamide resistance gene (sul1) having the highest rate (100%) and the polymyxin resistance gene (mcr-1) the lowest (3.6%).3. These isolates were validated to carry virulence genes pmfA, mrpA, atfC, rsbA, atfA, ureC and ucaA with the high prevalence of 96.4, 92.9, 92.0, 85.7, 85.7, 57.1 and 46.4%, respectively. Genotyping results using the ERIC-PCR indicated that the genetic similarity of all the isolates was 68.6% to 100% which fell into 4 clusters.4. The P. mirabilis isolates from the slaughterhouses exhibited higher levels of antibiotic resistance and a more pronounced MDR phenomenon than those from poultry farms and retail markets. The proportion of isolates carrying the most commonly detected resistant and virulence genes was higher in samples from poultry farms and slaughterhouses as opposed to retail markets. Importantly, there was genetic similarity and heterogeneity among P. mirabilis isolates from the three sources and genotypic diversity was the highest among isolates from retail markets, followed by slaughterhouses and poultry farms.