Osteoblastic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells on P3HT Thin Polymer Film.

IF 5.2 3区医学 Q1 ENGINEERING, BIOMEDICAL Journal of Functional Biomaterials Pub Date : 2025-01-02 DOI:10.3390/jfb16010010

Paola Campione, Maria Giovanna Rizzo, Luana Vittoria Bauso, Ileana Ielo, Grazia Maria Lucia Messina, Giovanna Calabrese

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Abstract

Bone defects restoration has always been an arduous challenge in the orthopedic field due to the limitations of conventional grafts. Bone tissue engineering offers an alternative approach by using biomimetic materials, stem cells, and growth factors that are able to improve the regeneration of bone tissue. Different biomaterials have attracted great interest in BTE applications, including the poly(3-hexylthiofene) (P3HT) conductive polymer, whose primary advantage is its capability to provide a native extracellular matrix-like environment. Based on this evidence, in this study, we evaluated the biological response of human adipose-derived mesenchymal stem cells cultured on P3HT thin polymer film for 14 days. Our results suggest that P3HT represents a good substrate to induce osteogenic differentiation of osteoprogenitor cells, even in the absence of specific inductive growth factors, thus representing a promising strategy for bone regenerative medicine. Therefore, the system provided may offer an innovative platform for next-generation biocompatible materials for regenerative medicine.

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P3HT薄膜对人脂肪源间充质干细胞成骨分化的影响。

由于传统移植物的局限性，骨缺损修复一直是骨科领域的一个艰巨挑战。骨组织工程提供了一种替代方法，通过使用仿生材料、干细胞和生长因子来改善骨组织的再生。不同的生物材料引起了人们对BTE应用的极大兴趣，包括聚（3-己基噻吩）（P3HT）导电聚合物，其主要优点是能够提供天然的细胞外基质样环境。基于这一证据，在本研究中，我们评估了人脂肪来源的间充质干细胞在P3HT薄聚合物膜上培养14天的生物学反应。我们的研究结果表明，即使在缺乏特定的诱导生长因子的情况下，P3HT也是诱导骨祖细胞成骨分化的良好底物，因此代表了骨再生医学的一个有希望的策略。因此，所提供的系统可以为下一代再生医学生物相容性材料提供创新平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Functional Biomaterials Engineering-Biomedical Engineering

CiteScore

4.60

自引率

4.20%

发文量

226

审稿时长

11 weeks

期刊介绍： Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.