Anti-Müllerian Hormone Enhances Migration and Invasion in Human Trophoblast Cells.

IF 1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY Annals of clinical and laboratory science Pub Date : 2024-11-01

Xuhua Hu, Depeng Zhao, Wanxue Wang, Shuhua Wu, Litong Zhu, Xuemei Li, Zhuomin Huang, Jilong Yao

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Abstract

Objective: Anti-Müllerian hormone (AMH) belongs to the transforming growth factor-β superfamily. Recent evidence shows that AMH and its type II receptor (AMHRII) are expressed by the placenta at term. The physiological role of AMH in trophoblast invasion and migration remains to be elucidated.

Methods: Immunofluorescence and western blot were used to detect the expression of AMH and AMHRII in trophoblast cell lines and chorionic-decidual mixture from early pregnancies. In vitro experiments, including siRNA transfection, transwell and wound healing assays were further performed to study the function of AMH in trophoblast cell line.

Results: AMH and AMHR2 are mainly expressed in the interstitial trophoblast (ITB) in human chorionic-decidual mixture of early pregnancy. We found that knocking down AMH resulted in decreased migration ability of trophoblast cells compared to the control group.

Conclusion: It is shown that AMH enhances trophoblast's invasion and migration abilities. AMH may play a role in embryo implantation and placenta formation.

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抗勒氏杆菌激素促进人滋养细胞迁移和侵袭。

目的：抗勒氏杆菌激素（AMH）属于转化生长因子-β超家族。最近的证据表明，AMH及其II型受体（AMHRII）在足月时由胎盘表达。AMH在滋养细胞侵袭和迁移中的生理作用仍有待阐明。方法：采用免疫荧光法和western blot法检测早孕人滋养细胞和绒毛膜-蜕膜混合物中AMH和AMHRII的表达。体外实验通过siRNA转染、transwell和创面愈合实验，进一步研究AMH在滋养细胞中的作用。结果：AMH和AMHR2主要表达于早孕人绒毛膜-蜕膜混合物的间质滋养细胞（ITB）中。我们发现，与对照组相比，敲除AMH导致滋养细胞迁移能力下降。结论：AMH能增强滋养细胞的侵袭和迁移能力。AMH可能在胚胎着床和胎盘形成中起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.