Structural modification of lipid membranes by polyphenols: A fluorescence spectroscopy study.

IF 2.8 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochimica et biophysica acta. Biomembranes Pub Date : 2025-01-22 DOI:10.1016/j.bbamem.2025.184411

Valeriya M Trusova, Uliana K Tarabara, Mette H Thomsen, Galyna P Gorbenko

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Abstract

The present study investigates the molecular mechanisms of polyphenol-lipid interactions and their impact on membrane properties. Using pyrene and DPH as reporter molecules, we examined the impact of quercetin, curcumin, gallic, and salicylic acids on membranes composed of phosphatidylcholine (PC) and its mixtures with phosphatidylglycerol (PG), cardiolipin (CL), and cholesterol (Chol). Quercetin was found to increase the lipid order without affecting the lipid bilayer free volume, indicating interactions near the membrane surface. In turn, curcumin exhibited more complex effects, reducing free volume in PC but increasing it in PG vesicles, reflecting its amphiphilic structure and variable penetration depth. Gallic and salicylic acids selectively increased free volume at the membrane core without influencing lipid order at the upper regions of lipid bilayer. The results obtained demonstrate that polyphenol structure and lipid composition dictate the resultant pattern of polyphenol-membranes interactions, which may have implications for drug delivery and nutraceutical design.

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来源期刊

Biochimica et biophysica acta. Biomembranes 生物-生化与分子生物学

CiteScore

8.20

自引率

5.90%

发文量

175

审稿时长

2.3 months

期刊介绍： BBA Biomembranes has its main focus on membrane structure, function and biomolecular organization, membrane proteins, receptors, channels and anchors, fluidity and composition, model membranes and liposomes, membrane surface studies and ligand interactions, transport studies, and membrane dynamics.