Jiaxin Qin, Ziqi Wang, Cheng Qian, Guohui Ji, Yizhuo Zhang, Zhanglei Cao, Bing Yan, Jun Cai
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引用次数: 0
Abstract
NupR is a nucleoside permease regulator belonging to the GntR family, mainly regulating nucleoside transport in Bacillus thuringiensis. A conserved binding site for NupR was found in the promoter region of plcR. This study aimed to investigate the regulation of the virulence regulator PlcR by NupR and its impact on Bt virulence. We demonstrated that NupR can directly repress the expression of plcR. The expression of plcR can be induced by glucose and nucleosides. Glucose impacts the expression of plcR mainly through Spo0A, while the induction effect of nucleosides may be due to the production of ribose through nucleoside catabolism. In addition, NupR regulates the expression of the PlcR regulon, including hemolysin, phospholipase C, papR, and oligopeptide permease, which could result in the culture supernatant of BMB171 being less virulent to sf9 cells compared to the nupR knockout strain. The results combine the nutritional status of cells with virulence to form a regulatory loop, providing new ideas and research foundations for the study of bacterial virulence.
期刊介绍：
Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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