Induction of sepsis in a rat model by the cecal ligation and puncture technique. Application for the study of experimental acute renal failure.

Abstract

Sepsis is a systemic inflammatory response to infection, and its occurrence is associated with a poor prognosis in the context of multiorgan dysfunction syndrome (MODS). Although there are several animal models for the study of its etiology, the cecal ligation and puncture (CLP) model has been considered the "Gold standard" because it shows a high degree of similarity to the progression of human sepsis. Currently, it is one of the most frequently chosen options to search for therapeutic alternatives to diminish the progression and organ damage induced by sepsis. Here, we describe in depth the CLP technique in a rat model and its application in the study of acute renal failure (ARF), the most severe complication during sepsis.