De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer Azohydromonas lata Strain H1 Genome and Genomic Analysis of PHB Production Machinery.

IF 4.2 2区生物学 Q2 MICROBIOLOGY Microorganisms Pub Date : 2025-01-10 DOI:10.3390/microorganisms13010137

Daniele Traversa, Carlo Pazzani, Pietro D'Addabbo, Lucia Trisolini, Matteo Chiara, Marta Oliva, Angelo Marzella, Camilla Mandorino, Carla Calia, Guglielmina Chimienti, Caterina Manzari, Graziano Pesole, Maria Scrascia

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Abstract

Polyhydroxybutyrate (PHB) is a biodegradable natural polymer produced by different prokaryotes as a valuable carbon and energy storage compound. Its biosynthesis pathway requires the sole expression of the phaCAB operon, although auxiliary genes play a role in controlling polymer accumulation, degradation, granule formation and stabilization. Due to its biodegradability, PHB is currently regarded as a promising alternative to synthetic plastics for industrial/biotechnological applications. Azohydromonas lata strain H1 has been reported to accumulate PHB by using simple, inexpensive carbon sources. Here, we present the first de novo genome assembly of the A. lata strain H1. The genome assembly is over 7.7 Mb in size, including a circular megaplasmid of approximately 456 Kbp. In addition to the phaCAB operon, single genes ascribable to PhaC and PhaA functions and auxiliary genes were also detected. A comparative genomic analysis of the available genomes of the genus Azohydromonas revealed the presence of phaCAB and auxiliary genes in all Azohydromonas species investigated, suggesting that the PHB production is a common feature of the genus. Based on sequence identity, we also suggest A. australica as the closest species to which the phaCAB operon of the strain H1, reported in 1998, is similar.

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聚羟基丁酸（PHB）产生菌偶氮单胞菌H1基因组的从头组装和PHB生产机制的基因组分析。

聚羟基丁酸酯（Polyhydroxybutyrate， PHB）是由不同的原核生物产生的一种可生物降解的天然聚合物，是一种有价值的碳和能量储存化合物。其生物合成途径需要单独表达phaCAB操纵子，尽管辅助基因在控制聚合物积累、降解、颗粒形成和稳定方面发挥作用。由于其可生物降解性，PHB目前被认为是工业/生物技术应用中合成塑料的有前途的替代品。据报道，偶氮单胞菌H1菌株通过使用简单、廉价的碳源积累PHB。在这里，我们提出了第一个新的基因组组装的A. lata菌株H1。基因组组装体的大小超过7.7 Mb，其中包括一个约456 Kbp的圆形巨质粒。除phaCAB操纵子外，还检测到PhaC和PhaA功能的单基因和辅助基因。对偶氮单胞菌属现有基因组的比较分析显示，在所调查的所有偶氮单胞菌种中都存在phaCAB和辅助基因，表明PHB的产生是该属的共同特征。基于序列同一性，我们还认为澳大利亚南澳州是与1998年报道的H1菌株的phaCAB操纵子相似的最接近的物种。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.