Exploring the Anti-Osteoporotic Effects of n-Hexane Fraction from Cotoneaster wilsonii Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo.

IF 4.8 3区医学 Q2 CHEMISTRY, MEDICINAL Pharmaceuticals Pub Date : 2025-01-03 DOI:10.3390/ph18010045

Soyeon Hong, Hee Ju Lee, Da Seul Jung, Saruul Erdenebileg, Hoseong Hwang, Hak Cheol Kwon, Jaeyoung Kwon, Gyhye Yoo

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Abstract

Background: Osteoporosis is characterized by the microstructural depletion of bone tissue and decreased bone density, leading to an increased risk of fractures. Cotoneaster wilsonii Nakai, an endemic species of the Korean Peninsula, grows wild in Ulleungdo. In this study, we aimed to investigate the effects of C. wilsonii and its components on osteoporosis.

Methods and results: The alkaline phosphatase (ALP) activity of C. wilsonii extracts and fractions was evaluated in MC3T3-E1 pre-osteoblasts, and the n-hexane fraction (CWH) showed the best properties for ALP activity. The effects of the CWH on bone formation were assessed in MC3T3-E1 cells and ovariectomized mice. Biochemical assays and histological analyses focused on the signaling activation of osteoblast differentiation and osteogenic markers, such as ALP, collagen, and osterix. The CWH significantly activated TGF-β and Wnt signaling, enhancing osteoblast differentiation and bone matrix formation. Notably, CWH treatment improved micro-CT indices, such as femoral bone density, and restored serum osteocalcin levels compared to OVX controls.

Conclusions: These results highlight the potential of the C. wilsonii Nakai n-hexane fraction as a promising therapeutic agent for managing osteoporosis.

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黄花冬正己烷部分抗骨质疏松作用的体内研究：激活Runx2和成骨细胞分化。

背景：骨质疏松症的特点是骨组织的微结构损耗和骨密度降低，导致骨折的风险增加。在郁陵岛野生生长着韩半岛特有的野生木。在本研究中，我们旨在研究巨噬菌及其成分对骨质疏松症的影响。方法与结果：以MC3T3-E1前成骨细胞为实验对象，测定其提取物及组分的碱性磷酸酶（ALP）活性，其中正己烷组分（CWH）的碱性磷酸酶活性最好。在MC3T3-E1细胞和去卵巢小鼠中评估CWH对骨形成的影响。生化分析和组织学分析侧重于成骨细胞分化和成骨标志物（如ALP、胶原和骨组织）的信号激活。CWH显著激活TGF-β和Wnt信号，促进成骨细胞分化和骨基质形成。值得注意的是，与OVX对照组相比，CWH治疗改善了微ct指标，如股骨骨密度，并恢复了血清骨钙素水平。结论：这些结果突出了C. wilsonii Nakai正己烷部分作为治疗骨质疏松症的有希望的治疗药物的潜力。

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来源期刊

Pharmaceuticals Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

6.10

自引率

4.30%

发文量

1332

审稿时长

6 weeks

期刊介绍： Pharmaceuticals (ISSN 1424-8247) is an international scientific journal of medicinal chemistry and related drug sciences.Our aim is to publish updated reviews as well as research articles with comprehensive theoretical and experimental details. Short communications are also accepted; therefore, there is no restriction on the maximum length of the papers.