Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1

IF 2.4 3区医学 Q3 VIROLOGY Virology Pub Date : 2025-01-20 DOI:10.1016/j.virol.2025.110402

Claudia Alejandra Kornuta , Juan Esteban Bidart , Ivana Soria , Valeria Quattrocchi , Mariela Gammella , María Virginia Tribulatti , Oscar Campetella , Cecilia Arahí Prato , Julieta Carabelli , Felipe Andrés Cheuquepán , Yanina Paola Hecker , Prando Dadin Moore , Patricia Inés Zamorano , Cecilia Ana Langellotti

{"title":"Galectin-8 and GEL01 as potential adjuvants to enhance the immune response induced by a DNA vaccine against bovine alphaherpesvirus Type-1","authors":"Claudia Alejandra Kornuta , Juan Esteban Bidart , Ivana Soria , Valeria Quattrocchi , Mariela Gammella , María Virginia Tribulatti , Oscar Campetella , Cecilia Arahí Prato , Julieta Carabelli , Felipe Andrés Cheuquepán , Yanina Paola Hecker , Prando Dadin Moore , Patricia Inés Zamorano , Cecilia Ana Langellotti","doi":"10.1016/j.virol.2025.110402","DOIUrl":null,"url":null,"abstract":"<div><div>Bovine alphaherpesvirus-1 (BoAHV-1) causes several symptoms in cattle, leading to significant costs for the livestock industry. In this study, we used a plasmid encoding a secreted form of BoAHV-1 glycoprotein D (pCIgD) as a DNA vaccine. To enhance the potency of the pCIgD vaccine, we used Montanide™ GEL01 PR (GEL01) and introduced Galectin-8 (Gal-8), a lectin considered a novel adjuvant due to its immunostimulatory effects, into the formulation.</div><div>Animals were vaccinated with pCIgD, pCIgD with Gal-8 (pCIgD-Gal-8), pCIgD with Gal-8 and GEL01 (pCIgD-Gal-8-GEL01), or the control plasmid pCIneo. The immune response was first assessed in a mouse model and then in bovines.</div><div>The results showed that combining Gal-8 and GEL01 with pCIgD modulated immune responses at both the humoral and cellular levels in both animal models.</div><div>This study evaluates the efficacy of a DNA vaccine with Gal-8 and GEL01 as potential adjuvants to enhance immune protection against BoAHV-1.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"604 ","pages":"Article 110402"},"PeriodicalIF":2.4000,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0042682225000145","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Bovine alphaherpesvirus-1 (BoAHV-1) causes several symptoms in cattle, leading to significant costs for the livestock industry. In this study, we used a plasmid encoding a secreted form of BoAHV-1 glycoprotein D (pCIgD) as a DNA vaccine. To enhance the potency of the pCIgD vaccine, we used Montanide™ GEL01 PR (GEL01) and introduced Galectin-8 (Gal-8), a lectin considered a novel adjuvant due to its immunostimulatory effects, into the formulation.

Animals were vaccinated with pCIgD, pCIgD with Gal-8 (pCIgD-Gal-8), pCIgD with Gal-8 and GEL01 (pCIgD-Gal-8-GEL01), or the control plasmid pCIneo. The immune response was first assessed in a mouse model and then in bovines.

The results showed that combining Gal-8 and GEL01 with pCIgD modulated immune responses at both the humoral and cellular levels in both animal models.

This study evaluates the efficacy of a DNA vaccine with Gal-8 and GEL01 as potential adjuvants to enhance immune protection against BoAHV-1.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

半乳糖凝集素-8和GEL01作为潜在佐剂增强牛1型α疱疹病毒DNA疫苗诱导的免疫应答

牛甲疱疹病毒-1 （BoAHV-1）在牛中引起几种症状，给畜牧业造成重大损失。在这项研究中，我们使用编码BoAHV-1糖蛋白D （pCIgD）分泌形式的质粒作为DNA疫苗。为了增强pCIgD疫苗的效力，我们使用Montanide™GEL01 PR (GEL01)，并在配方中引入半乳糖凝集素-8 (Gal-8)，一种因其免疫刺激作用而被认为是新型佐剂的凝集素。分别接种pCIgD、pCIgD-Gal-8 （pCIgD-Gal-8）、pCIgD-Gal-8-GEL01 （pCIgD-Gal-8-GEL01）或对照质粒pCIneo。免疫反应首先在小鼠模型中进行了评估，然后在牛身上进行了评估。结果表明，在两种动物模型中，Gal-8和GEL01与pCIgD结合可在体液和细胞水平上调节免疫反应。本研究评估了以Gal-8和GEL01作为潜在佐剂的DNA疫苗增强对BoAHV-1的免疫保护的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.