Real-Time 5-Hydroxyindoleacetic Acid Monitoring in Guinea Pig Brain Using a Molecular Imprinted Polymer-Based Galvanic Redox Potentiometric Sensor

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2025-01-27 DOI:10.1021/acs.analchem.4c04990
Yuxin Peng, Fenghui Zhu, Huan Wei, Jiping Ni, Xin Yang, Yichun Yao, Ying Jiang
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Abstract

5-Hydroxyindoleacetic acid (5-HIAA), a vital metabolite of serotonin (5-HT), is crucial for understanding metabolic pathways and is implicated in various mental disorders. In situ monitoring of 5-HIAA is challenging due to the lack of affinity ligands and issues with electrochemical fouling. We present an advanced sensing approach that integrates customizable molecular imprinting polymer (MIP) with self-driven galvanic redox potentiometry (GRP) for precise, real-time in vivo monitoring of 5-HIAA. The sensor, featuring pyrrole as the functional monomer in the MIP on the micrometer-sized bipolar carbon fiber electrodes, exhibited nanomolar sensitivity and superior selectivity for 5-HIAA over biosynthetic pathway analogs like 5-hydroxytryptophan (5-HTP) and serotonin. The MIPGRP sensor demonstrated excellent reversibility and resistance to fouling, enabling continuous monitoring in live guinea pig brains. We observed that intraperitoneal 5-HTP injection increases brain 5-HIAA levels, which is amplified up to 8-fold with Carbidopa pretreatment, providing deeper insights into the serotonergic signaling pathway. This work underscores the MIPGRP sensor’s potential as a versatile and reliable tool for advancing neuroscience research.

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利用基于分子印迹聚合物的电化学氧化还原电位传感器实时监测豚鼠大脑中的 5-羟基吲哚乙酸
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文献相关原料
公司名称
产品信息
索莱宝
Serotonin hydrochloride
索莱宝
L-tryptophan
索莱宝
Bovine serum albumin (BSA)
阿拉丁
Perchlorate (LiClO4)
来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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