AKT activation participates in Fascin-1-induced EMT in hepatoma cells.

IF 1.7 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Cytotechnology Pub Date : 2025-04-01 Epub Date: 2025-01-25 DOI:10.1007/s10616-025-00707-9

Pengju Zhao, Kewei Ai, Yi Li, Wei Cheng, Jiwu Yang

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Abstract

High expression of Fascin-1 involves high metastasis, high recurrence, and poor prognosis of cancers. However, the related regulatory mechanism in hepatocellular carcinoma (HCC) remains elusive. In this study, Fascin-1 was highly expressed in HCC tissues and cell lines. Fastin-1 protein levels and p-Akt1/Akt1 rate were increased by Akt activator SC79 and were decreased by Akt inhibitor LY294002. Silenced Fascin-1 suppressed cell proliferation, promoted cell apoptosis, suppressed cell invasion and epithelial-mesenchymal transition (EMT) in HCC cell lines. Also, silenced Fascin-1 induced cell cycle arrest in the G1 phase. Moreover, silenced Fascin-1 repressed invasion of HCC cells by inhibiting EMT. Besides, interference with Fascin-1 inhibited HCC cell growth, reduced Vimentin expressions and p-Akt1/Akt1 rate in vivo, while these impacts were abolished after injection of SC79. In conclusion, silencing Fascin-1 reduced the malignant growth of HCC, and this process was closely related to AKT inactivation.

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-025-00707-9.

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AKT激活参与了fastin -1诱导的肝癌细胞EMT。

fasin -1的高表达与肿瘤的高转移、高复发、预后差有关。然而，肝细胞癌（HCC）的相关调控机制尚不清楚。本研究中，fasin -1在HCC组织和细胞系中高表达。Akt激活剂SC79增加了fatin -1蛋白水平和p-Akt1/Akt1比率，Akt抑制剂LY294002降低了fatin -1蛋白水平。沉默的fasin -1抑制HCC细胞系细胞增殖，促进细胞凋亡，抑制细胞侵袭和上皮间质转化（EMT）。此外，沉默的fasin -1诱导细胞周期阻滞在G1期。此外，沉默的fastin -1通过抑制EMT抑制HCC细胞的侵袭。此外，干扰fastin -1抑制HCC细胞生长，降低体内Vimentin表达和p-Akt1/Akt1比率，而注射SC79后这些影响被消除。综上所述，沉默fasin -1可降低HCC的恶性生长，这一过程与AKT失活密切相关。补充信息：在线版本包含补充资料，可在10.1007/s10616-025-00707-9获得。

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.