Evaluation of l-cell activity in the small intestine according to the extension of the biliopancreatic loop in patients undergoing Roux-en-Y gastric by-pass

IF 2.4 4区医学 Q2 MEDICINE, GENERAL & INTERNAL Clinics Pub Date : 2025-01-01 DOI:10.1016/j.clinsp.2024.100555

Priscila Costa Estabile, Márcia Saldanha Kubrusly, Robson Kiyoshi Ishida, André Bubna Hirayama, Roberto de Cleva, Marco Aurelio Santo

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Abstract

Background

Individuals with severe obesity and type 2 diabetes mellitus have reduced secretion of incretins by L cells. Studies suggest an increase in L cell activity according to the length of the Biliopancreatic Loop (BPL).

Objective

Compare the effect of biliopancreatic loop extension on the number and expression of L cells in patients undergoing RYGB

Methods

Subjects (n = 13) undergoing RYGB with a BPL of 100 cm (G1) or 200 cm (G2). Intestinal biopsies were done before (T1) and 6 months after (T2) RYGB in 3 segments: gastro-enteric anastomosis (A), entero-enteric anastomosis (B) and terminal ileum (C). Analyzes of intestinal biopsies by immunohistochemistry and qRT-PCR.

Results

There was an increase (p < 0.0001) in L cells marked by PYY and GLP1 between T1 (17 ± 10.5) and T2 (23.5 ± 10.7) only at point C. There was no difference in L cells expression between groups G1 and G2 at points A (A1: 17.3 ± 2.9; A2: 19.9 ± 1.9; p = 0.09), B (B1: 13.7 ± 6.6; B2: 14.1 ± 4.9; p = 0.89) and C (C1: 13.2 ± 2.2; C2: 11.4 ± 3.4; p = 0.32) in PYY and GLP1 gene expression (A1: 20.8 ± 4, 1; A2: 23.7 ± 3.6; p = 0.2), B (B1:14.3 ± 7.9; B2: 22.7 ± 11.8; p = 0.1), (C1: 17±4.1; C2: 21.1 ± 4.8; p = 0.2).

Conclusions

Both techniques lead to an increase in the number of active L cells.

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根据Roux-en-Y胃旁路术患者胆胰袢延伸评估小肠l细胞活性。

背景：重度肥胖和2型糖尿病患者的L细胞分泌肠促胰岛素减少。研究表明，L细胞活性的增加与胆胰环（BPL）的长度有关。目的：比较胆胰袢延长对RYGB患者L细胞数量和表达的影响。方法：BPL为100 cm （G1）和200 cm （G2）的RYGB患者（n = 13）。在RYGB术前（T1）和术后（T2） 6个月分别对胃-肠吻合处(A)、肠-肠吻合处(B)和回肠末端(C) 3段进行肠道活检。采用免疫组织化学和qRT-PCR对肠道活检结果进行分析。结果：T1组（17±10.5）与T2组（23.5±10.7）仅在c点PYY和GLP1标记的L细胞表达量升高（p < 0.0001）， G1组与G2组在A点L细胞表达量差异无统计学意义(A1: 17.3±2.9；A2: 19.9±1.9；p = 0.09), B (B1: 13.7±6.6；B2: 14.1±4.9；p = 0.89)和C (C1: 13.2±2.2；C2: 11.4±3.4；p = 0.32)在PYY和GLP1基因表达中的差异(A1: 20.8±4,1；A2: 23.7±3.6；p = 0.2), B (B1:14.3±7.9；B2: 22.7±11.8；p = 0.1), (C1: 17±4.1；C2: 21.1±4.8；P = 0.2)。结论：两种方法均可增加活性L细胞的数量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinics 医学-医学：内科

CiteScore

4.10

自引率

3.70%

发文量

129

审稿时长

52 days

期刊介绍： CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.