Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoring

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES MethodsX Pub Date : 2024-12-31 DOI:10.1016/j.mex.2024.103133

Nurul Affiqah Edora Mohd-Azhar , Haslawati Baharuddin , Mohamad-Sufiyan Salmi , Noor Faizah Ismail , Amatul-Samahah Md. Ali , Syazwan Saidin , Adibah Abu-Bakar

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Abstract

The Asian Arowana, Scleropages formosus (Müller and Schlegel, 1844) is a large majestic freshwater teleost, crowned as the king of aquariums with its bright charismatic appearance and magnificent swimming performance. The most expensive Asian arowana is the Golden Blue-based Malayan Arowana which is endemic to the Bukit Merah Lake and Kerian River Basin, Perak, Malaysia. S. formosus has been listed as endangered by the IUCN (International Union for Conservation of Nature), regulated under Appendix 1 of the Convention of International Trade on Endangered Species (CITES) for commercial trade. Environmental DNA (eDNA) analysis has become widely accepted in biodiversity monitoring for the detection of rare and endangered species without harming any ecosystem or threatened species. Hence, the application of eDNA as wild population monitoring of S. formosus is possible for conservation and CITES enforcement program.

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The species-specific primer of S. formosus was designed based on selected sequences obtained from GenBank
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This report presents the potential application of eDNA in the management of the Malaysian 686 CITES Act for conservation monitoring of the Asian Arowana
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The detection and wild population monitoring is possible through the eDNA method as complementary tools

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濒危亚洲龙鱼物种特异性环境DNA （eDNA）引物的开发和验证，用于执法和野生种群监测。

亚洲龙鱼，sccleropages formosus （m ller and Schlegel, 1844）是一种大型的淡水硬骨鱼，以其明亮迷人的外表和华丽的游泳表演被誉为水族馆之王。最昂贵的亚洲龙鱼是金蓝色的马来亚龙鱼，它是马来西亚霹雳州武吉美拉湖和克里安河流域的特有品种。台湾梧桐已被国际自然保护联盟列为濒危物种，受《濒危物种国际贸易公约》（CITES）附录1管制，可进行商业贸易。环境DNA （Environmental DNA, eDNA）分析在生物多样性监测中已被广泛接受，可以在不损害任何生态系统或受威胁物种的情况下检测稀有和濒危物种。因此，利用eDNA技术对台湾白檀进行野生种群监测，为台湾白檀的保护和CITES执法提供了可能。•根据从GenBank获得的精选序列设计了S. formosus的物种特异性引物•本报告提出了eDNA在马来西亚686 CITES法案管理中用于亚洲龙鱼保护监测的潜在应用•通过eDNA方法作为补充工具可以进行检测和野生种群监测。

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