Fabrication of water-dispersible dye/polymer matrix-stabilized β-FeOOH (Rh-B/F127@β-FeOOH) nanoparticles: synthesis, characterization and therapeutic applications

Abstract

In this study, dye/polymer matrix-stabilized β-FeOOH nanomaterials were fabricated for therapeutic applications. Rh-B/F127@β-FeOOH nanomaterials were synthesized using two different methods: co-precipitation (CoP) and hydrothermal (HT) methods. The as-synthesized nanoparticles were characterized using various spectroscopic techniques, including FT-IR, UV-Vis, PL, XRD, HR-TEM, and XPS analysis. The functional groups and optical properties were confirmed by FT-IR spectroscopy, UV-Vis and fluorescence spectroscopy. The Rh-B/F127@β-FeOOH nanomaterials exhibited both rod-like and sphere-like morphology, as confirmed by HR-TEM analysis. Unlike the nanorods, the nanospheres produced multi-colored emissions at 407, 446, 482 and 520 nm. The oxidative states and elements were confirmed by XPS spectroscopy. MTT assays were used to analyze the cytotoxicity of the nanospheres against A549 cells. The reactive oxygen species (ROS) generation and apoptotic cell death caused by the β-FeOOH nanospheres were evaluated by flow cytometry. Cell cycle analysis indicated that the treatment of nanospheres-induced S-phase cell cycle arrest in A549 cells. The synthesized nanospheres induced late-stage apoptosis in the A549 cell line, with a cell death rate of up to 30.37% at the IC₅₀ concentration. Additionally, the antioxidant activities of the synthesized nanorods showed a high scavenging activity against free radicals, as examined by different assays such as such as DPPH, RP, and FRAP. The above results suggest that the synthesized nanorods and nanospheres are promising and efficient material for therapeutic applications.