Characterization of 3D printed micro-blades for cutting tissue-embedding material

IF 4.3 3区 工程技术 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY Extreme Mechanics Letters Pub Date : 2025-01-01 DOI:10.1016/j.eml.2024.102288
Saisneha Koppaka, David Doan, Wei Cai, X. Wendy Gu, Sindy K.Y. Tang
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引用次数: 0

Abstract

Cutting soft materials on the microscale has emerging applications in single-cell studies, tissue microdissection for organoid culture, drug screens, and other analyses. However, the cutting process is complex and remains incompletely understood. Furthermore, precise control over blade geometries, such as the blade tip radius, has been difficult to achieve. In this work, we use the Nanoscribe 3D printer to precisely fabricate micro-blades (i.e., blades <1 mm in length) and blade grid geometries. This fabrication method enables a systematic study of the effect of blade geometry on the indentation cutting of paraffin wax, a common tissue-embedding material. First, we print straight micro-blades with tip radius ranging from ∼100 nm to 10 μm. The micro-blades are mounted in a custom nanoindentation setup to measure the cutting energy during indentation cutting of paraffin. Cutting energy, measured as the difference in dissipated energy between the first and second loading cycles, decreases as blade tip radius decreases, until ∼357 nm when the cutting energy plateaus despite further decrease in tip radius. Second, we expand our method to blades printed in unconventional configurations, including parallel blade structures and blades arranged in a square grid. Under the conditions tested, the cutting energy scales approximately linearly with the total length of the blades comprising the blade structure. The experimental platform described can be extended to investigate other blade geometries and guide the design of microscale cutting of soft materials.
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来源期刊
Extreme Mechanics Letters
Extreme Mechanics Letters Engineering-Mechanics of Materials
CiteScore
9.20
自引率
4.30%
发文量
179
审稿时长
45 days
期刊介绍: Extreme Mechanics Letters (EML) enables rapid communication of research that highlights the role of mechanics in multi-disciplinary areas across materials science, physics, chemistry, biology, medicine and engineering. Emphasis is on the impact, depth and originality of new concepts, methods and observations at the forefront of applied sciences.
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