Small-Angle and Quasi-Elastic Neutron Scattering from Polydisperse Oligolamellar Vesicles Containing Glycolipids

IF 4.6 2区化学 Q2 CHEMISTRY, PHYSICAL The Journal of Physical Chemistry Letters Pub Date : 2025-01-28 DOI:10.1021/acs.jpclett.4c03454

Lukas Bange, Amin Rahimzadeh, Tetiana Mukhina, Regine von Klitzing, Ingo Hoffmann, Emanuel Schneck

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Abstract

Glycolipids are known to stabilize biomembrane multilayers through preferential sugar–sugar interactions that act as weak transient membrane cross-links. Here, we use small-angle and quasi-elastic neutron scattering on oligolamellar phospholipid vesicles containing defined glycolipid fractions in order to elucidate the influence of glycolipids on membrane mechanics and dynamics. Small-angle neutron scattering (SANS) reveals that the oligolamellar vesicles (OLVs) obtained by extrusion are polydisperse with regard to the number of lamellae, n, which renders the interpretation of the quasi-elastic neutron spin echo (NSE) data nontrivial. To overcome this problem, we propose a method to model the NSE data in a rigorous fashion based on the obtained histograms of n and on their q-dependent intensity-weighted contribution. This procedure yields meaningful values for the bending rigidity of individual lipid membranes and insights into the mechanical coupling between adjacent membrane lamellae including the effect of the glycolipids.

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含糖脂的多分散低聚层状囊泡的小角和准弹性中子散射

已知糖脂通过优先的糖-糖相互作用作为弱瞬态膜交联来稳定生物膜多层。为了阐明糖脂对膜力学和动力学的影响，我们利用小角度和准弹性中子散射对含有特定糖脂组分的低聚层磷脂囊泡进行了研究。小角中子散射（SANS）表明，挤压得到的寡层状囊泡（OLVs）在片层数n方面是多分散的，这使得准弹性中子自旋回波（NSE）数据的解释具有重要意义。为了克服这个问题，我们提出了一种方法，基于获得的n的直方图及其依赖于q的强度加权贡献，以严格的方式对NSE数据进行建模。这一过程为单个脂质膜的弯曲刚度提供了有意义的值，并深入了解了相邻膜片之间的机械耦合，包括糖脂的影响。

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来源期刊

The Journal of Physical Chemistry Letters CHEMISTRY, PHYSICAL-NANOSCIENCE & NANOTECHNOLOGY

CiteScore

9.60

自引率

7.00%

发文量

1519

审稿时长

1.6 months

期刊介绍： The Journal of Physical Chemistry (JPC) Letters is devoted to reporting new and original experimental and theoretical basic research of interest to physical chemists, biophysical chemists, chemical physicists, physicists, material scientists, and engineers. An important criterion for acceptance is that the paper reports a significant scientific advance and/or physical insight such that rapid publication is essential. Two issues of JPC Letters are published each month.