Description of six novel species Bacillus bruguierae sp. nov., Bacillus kandeliae sp. nov., Bacillus yunxiaonensis sp. nov., Metabacillus rhizosphaerae sp. nov., Metabacillus sediminis sp. nov. and Psychrobacillus mangrovi sp. nov., isolated from mangrove ecosystem.

IF 2 3区生物学 Q4 MICROBIOLOGY International journal of systematic and evolutionary microbiology Pub Date : 2025-01-01 DOI:10.1099/ijsem.0.006656

Guo-Hong Liu, Manik Prabhu Narsing Rao, Huai Shi, Qian-Qian Chen, Syed Raziuddin Quadri, Wen-Jun Li

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Abstract

Six Gram-stain-positive and rod-shaped strains, designated FJAT-51614^T, FJAT-51639^T, FJAT-52054^T, FJAT-52991^T, FJAT-53654^T and FJAT-53711^T, were isolated from a mangrove ecosystem. The condition for growth among the strains varied (pH ranging 5.0-11.0 and temperature 15-45 °C). These strains showed the highest 16S rRNA gene sequence similarity to the genera Bacillus, Metabacillus and Psychrobacillus. Menaquinone MK-7 (100%) was present in all strains except strain FJAT-51614^T, which contained MK-7 (32%) and MK-8 (68%). The major fatty acids (>10%) in strains FJAT-52054^T and FJAT-51614^T were anteiso-C_15 : 0 and iso-C_14 : 0. The major fatty acid (>10%) in strain FJAT-53654^T was anteiso-C_15 : 0, while in strain FJAT-51639^T were iso-C_15 : 0 and iso-C_17 : 0. The major fatty acids in strains FJAT-52991^T and FJAT-53711^T were iso-C_15 : 0 and iso-C_16 : 0. The major polar lipids in strains FJAT-51639^T, FJAT-52991^T and FJAT-51614^T were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, while strains FJAT-53711^T, FJAT-52054^T and FJAT-53654^T consist of diphosphatidylglycerol and phosphatidylglycerol. The genome-relatedness values among the present study strains and the closest species were below the recognized cutoff levels for species delineation. The polyphasic analysis comparison results represent six strains as novel species for which the names Bacillus bruguierae sp. nov. (FJAT-51639^T=GDMCC 1.3073^T=JCM 35615^T), Bacillus kandeliae sp. nov. (FJAT-52991^T=GDMCC 1.3069^T=JCM 35619^T), Bacillus yunxiaonensis sp. nov. (FJAT-53711^T=GDMCC 1.3068^T=JCM 35616^T), Metabacillus rhizosphaerae sp. nov. (FJAT-53654^T=GDMCC 1.3075^T=JCM 35614^T), Metabacillus sediminis sp. nov. (FJAT-52054^T=GDMCC 1.3074^T=JCM 35620^T) and Psychrobacillus mangrovi sp. nov. (FJAT-51614^T=GDMCC 1.3072^T=JCM35478^T) are proposed.

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从红树林生态系统中分离出六株革兰氏染色阳性的杆状菌株，分别命名为 FJAT-51614T、FJAT-51639T、FJAT-52054T、FJAT-52991T、FJAT-53654T 和 FJAT-53711T。这些菌株的生长条件各不相同（pH 值在 5.0-11.0 之间，温度为 15-45 ℃）。这些菌株的 16S rRNA 基因序列与芽孢杆菌属、拟杆菌属和精神杆菌属的相似度最高。除菌株 FJAT-51614T 含有 MK-7（32%）和 MK-8（68%）外，其他菌株均含有 Menaquinone MK-7（100%）。FJAT-52054T 和 FJAT-51614T 菌株中的主要脂肪酸（>10%）是前异-C15 ：0 和异-C14 ：0.菌株 FJAT-53654T 的主要脂肪酸（>10%）是前异-C15 ：0，而在菌株 FJAT-51639T 中则是异-C15 : 0 和异-C17 : 0：0 和异 C17 ：0.菌株 FJAT-52991T 和 FJAT-53711T 的主要脂肪酸是异-C15 : 0 和异-C16 : 0：0 和异 C16 ：0.菌株 FJAT-51639T、FJAT-52991T 和 FJAT-51614T 的主要极性脂质为二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺，而菌株 FJAT-53711T、FJAT-52054T 和 FJAT-53654T 则由二磷脂酰甘油和磷脂酰甘油组成。本研究菌株与最接近物种之间的基因组相关性值低于公认的物种划分临界值。多相分析比较结果表明，6 株菌株为新种，其名称分别为 Bacillus bruguierae sp.nov.（FJAT-51639T=GDMCC 1.3073T=JCM 35615T）、Bacillus kandeliae sp.nov.（FJAT-52991T=GDMCC 1.3069T=JCM 35619T）、Bacillus yunxiaonensis sp.nov.（FJAT-53711T=GDMCC 1.3069T=JCM 35619T）、Bacillus yunxiaonensis sp.(FJAT-53711T=GDMCC 1.3068T=JCM 35616T), Metabacillus rhizosphaerae sp.nov.（FJAT-52054T=GDMCC 1.3074T=JCM 35620T）和 Psychrobacillus mangrovi sp.

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.