Ana Cristina Padilha Janini, Brenda Fornazaro Moraes, Lauter Eston Pelepenko, Victor Augusto Benedicto Dos Santos, Matheus Barros-Costa, Gabriela Fernanda Malosá, Fábio Roberto de Souza Batista, Juliana de Aguiar Silveira Meira, Mariza Akemi Matsumoto, Thiago Bessa Marconato Antunes, Gaspar Darin Filho, Francisco Haiter Neto, Brenda Paula Figueiredo de Almeida Gomes, Marina Angélica Marciano
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引用次数: 0
Abstract
Objectives: To investigate volumetric changes, in vivo biocompatibility, and systemic migration from eight commercial endodontic sealer materials in paste/paste, powder/liquid, and pre-mixed forms.
Materials and methods: The sealers AH Plus Bioceramic, AH Plus Jet, BioRoot RCS, MTApex, Bio-C Sealer, Bio-C Sealer Ion+, EndoSequence BC Sealer and NeoSEALER Flo were studied. After characterisation by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), Raman spectroscopy and X-ray diffractometry (XRD), tubes were implanted in Wistar rats' alveolar bone and subcutaneous tissues. Micro-CT evaluated volumetric changes pre/post 30 days of implantation. Histological and immunohistochemistry analyses assessed biocompatibility. Kidney samples underwent spectrometry (ICP-MS) for tantalum, tungsten and zirconium. Statistical analysis determined normality and significance (udp < 0.05).
Results: Characterisation revealed calcium, silicon, and radiopacifiers in the materials. Volumetric changes showed greater alteration in subcutaneous tissues than alveolar bone; BioRoot RCS and MTApex (powder/liquid) were most stable. Histological analysis indicated intense inflammation for AH Plus Jet, moderate for others; IL-10 was marked positively for all materials. AH Plus Jet had an 18-fold higher tungsten and a 37-fold higher zirconium mass fraction in kidneys versus controls, while tantalum showed lower accumulation patterns.
Conclusion: Root canal filling materials' responses varied by implantation site and form, demonstrating acceptable biocompatibility. Tantalum and zirconium oxide radiopacifiers appear systemically safe; tungsten-based radiopacifiers are unsuitable due to metal accumulation risks.
Clinical relevance: This study highlights the need for further in vivo studies on endodontic sealers' chemical, biological, and physical behaviors and their systemic migration.
目的：研究膏状/膏状、粉状/液体和预混合形式的8种牙髓密封材料的体积变化、体内生物相容性和系统迁移。材料与方法：对AH Plus Bioceramic、AH Plus Jet、BioRoot RCS、MTApex、Bio-C Sealer、Bio-C Sealer Ion+、EndoSequence BC Sealer和NeoSEALER Flo进行了研究。采用扫描电镜（SEM）、x射线能谱（EDS）、拉曼光谱（Raman）和x射线衍射（XRD）对其进行表征，并将其植入Wistar大鼠的牙槽骨和皮下组织中。Micro-CT评估植入前后30天的体积变化。组织和免疫组织化学分析评估生物相容性。肾标本采用ICP-MS测定钽、钨和锆。统计分析确定了正态性和显著性（udp）结果：表征显示材料中有钙、硅和放射性不透明物质。体积变化显示皮下组织比牙槽骨变化更大；BioRoot RCS和MTApex（粉末/液体）最稳定。组织学分析显示AH Plus Jet患者炎症剧烈，其他患者炎症中度；IL-10在所有材料中均呈阳性。与对照组相比，AH Plus Jet在肾脏中的钨质量分数高18倍，锆质量分数高37倍，而钽的积累模式较低。结论：根管充填材料在不同种植位置和形态下的反应不同，具有良好的生物相容性。钽和氧化锆放射性不浊剂似乎是全身安全的；由于金属积累的风险，钨基放射性不浊剂不适合使用。临床意义：本研究强调了对牙髓密封剂的化学、生物和物理行为及其全身迁移进行进一步体内研究的必要性。
期刊介绍：
The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.
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