Systematic Profile of Oxylipins in Myocardial Infarction by Liquid Chromatography–Tandem Mass Spectrometry

IF 1.7 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS Rapid Communications in Mass Spectrometry Pub Date : 2025-01-30 DOI:10.1002/rcm.9999

Xin Tan, Hui Ni, Qin Li, Zhanglin Ma

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Abstract

Rationale

Oxylipins play an important role in inflammatory processes, accompanying the occurrence of myocardial infarction. Analyzing a wide panel of oxylipins derived from more polyunsaturated fatty acids may provide valuable information to elucidate the relationships between the signaling mediator profile and myocardial infarction comprehensively.

Methods

An ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for simultaneously measuring 74 oxylipins in 50-μL human samples with a 10-min chromatographic run and easy operation has been developed and validated.

Results

Accuracy and precision were below 15% of the relative errors in 99% of quality control. Recoveries and matrix effect were considered acceptable. Potential effects of different collecting tubes were also assessed. We successfully utilized our approach to measure plasma samples obtained from 99 healthy individuals and 302 myocardial infarction patients.

Conclusions

Profiles of oxylipins discovered potential biomarkers and clarified the pathological characteristics of oxylipin metabolism in myocardial infarction. Our approach was rapid, accurate, and precise, with high throughput, low sample volume, and easy operation, suitable for large-scale studies.

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液相色谱-串联质谱法测定心肌梗死中氧脂类的系统谱。

理由：氧化脂素在炎症过程中起重要作用，伴随心肌梗死的发生。分析来自更多多不饱和脂肪酸的广泛的氧化脂类可能为全面阐明信号介质谱与心肌梗死之间的关系提供有价值的信息。方法：建立了一种高效液相色谱-串联质谱（UHPLC-MS/MS）同时测定50 μ l人体样品中74种氧脂类的方法，色谱运行时间为10 min，操作简便。结果：准确度和精密度在99%的质量控制相对误差的15%以下。回收率和基质效应可接受。并对不同收集管的潜在影响进行了评价。我们成功地利用我们的方法测量了99名健康人和302名心肌梗死患者的血浆样本。结论：氧脂素谱发现了潜在的生物标志物，阐明了心肌梗死时氧脂素代谢的病理特征。我们的方法快速、准确、精确，具有高通量、低样本量、操作方便等特点，适合大规模研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.