The Association Between Adiponectin Gene Polymorphism (rs1501299) and Metabolic Syndrome.

Abstract

Objective: To investigate the allelic genotypes of the adiponectin (APN) gene polymorphisms (rs1501299) and its association with APN level among Mets patients.

Methods: A total of 410 patients with Mets and 203 healthy subjects were included in the study. The serum APN levels of the subjects were detected using enzyme-linked immunosorbent assay. The polymorphisms of the G/T gene at the rs1501299 locus of the APN gene were detected using restriction fragment length polymorphism polymerase chain reaction technology.

Results: The serum APN levels were significantly lower in Mets patients than in the control group (15.0 ± 4.9 mg/L vs 27.2 ± 6.5 mg/L, p < 0.05). The distribution of the three genotypes at the rs1501299 locus was statistically different between the Mets patients and the control group (GG, GT, and TT, p < 0.05), and the frequencies of the T alleles were higher in the Mets patients than in control group (GT and TT, p < 0.05). Logistic regression analysis showed that the study subjects with the T allele had a higher risk of Mets than those with the G allele (OR = 1.85, p < 0.05). The risk of Mets was higher in GT and TT genotypes compared to in GG genotypes (OR = 1.43; OR = 2.14 vs OR = 1.00 ref). Similarly, it increased after combining GT and GG genotypes (OR = 1.73, p < 0.05). The APN levels in the GT (14.3 ± 5.3 mg/L) and TT (13.4 ± 5.4 mg/L) genotypes of the study subjects were lower than those of the GG genotype (15.5 ± 4.8 mg/L, p < 0.05).

Conclusion: The occurrence of Mets may be associated with genetic variants at the rs1501299 locus, especially for individuals with G to T variants that reduce APN levels and lead to a higher risk of developing Mets.