WITHDRAWN: Retinal Phenotypes and Single-cell Sequencing Analysis of Ush2a Knockout Mice.

IF 2.7 2区 医学 Q1 OPHTHALMOLOGY Experimental eye research Pub Date : 2025-01-28 DOI:10.1016/j.exer.2025.110247
Yudie Ning, Longhao Kuang, Tao Huang, JunFeng Lv, Xiaohe Yan
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Abstract

This article has been withdrawn at the request of the editor due to an error in the publishing process. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/policies-and-standards/article-withdrawal.

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Ush2a基因敲除小鼠视网膜表型和单细胞测序分析。
Usher综合征是一种罕见的常染色体隐性遗传疾病,主要影响视力和听力,表现为视网膜色素变性引起的感觉神经性听力丧失和进行性视力丧失。Usher综合征视网膜变性的发病机制在很大程度上仍然未知。本研究利用CRISPR/Cas9技术成功构建了新型Ush2a敲除小鼠模型。对Ush2a敲除小鼠和野生型小鼠进行听觉脑干反应测试、视网膜电图、HE染色和视网膜单细胞RNA测序。Ush2a基因敲除小鼠的初始表型观察显示,Ush2a基因敲除小鼠在6个月时出现听觉功能障碍,但在20个月时未发现明显的视网膜形态和电生理表型。通过单细胞RNA测序了解Ush2a基因敲除小鼠视网膜中不同细胞类型的表达谱,构建初始单细胞调控图谱。我们的研究初步阐明了ush2a相关分子的可能机制和细胞发育过程中潜在的调控程序。
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来源期刊
Experimental eye research
Experimental eye research 医学-眼科学
CiteScore
6.80
自引率
5.90%
发文量
323
审稿时长
66 days
期刊介绍: The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.
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