CCDC154 knockdown inhibits growth of liver cancer via suppressing expression of Snail.

IF 3.4 3区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL European Journal of Medical Research Pub Date : 2025-01-30 DOI:10.1186/s40001-025-02290-3

Rulan Ma, Hongmei Nie, Caijing Mo, Dawei Yuan, Kun Zhu, Kang Li

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引用次数: 0

Abstract

Objective: The effect of coiled-coil domain-containing 154 (CCDC154) in liver cancer (LC) remains unexplored. The objective of this study was to investigate the role of CCDC154 in LC and its underlying mechanism.

Methods: The analysis of CCDC154 expression and prognosis was performed using UALCAN, Human Protein Atlas and Kaplan-Meier plotter websites. Protein expression was measured using Western blotting assay. Lentivirus was used to silence CCDC154 expression in LC cells. The proliferation and apoptosis of LC cells was evaluated by cell counting assay, colony formation assay and flow cytometry. The migration and invasion of LC cells were investigated using scratch wound-healing assay and Transwell assay.

Results: The results showed that CCDC154 was highly expressed in LC and related to tumor grade and stage. High CCDC154 expression was associated with to poor outcomes in LC patients. Silencing of CCDC154 inhibited proliferation, migration and invasion of LC cells. It also increased apoptosis in LC cells. After CCDC154 knockdown, the expression of Twist, Vimentin and Snail was down-regulated. Overexpression of Snail abated the inhibitory caused by CCDC154 knockdown on LC cell growth.

Conclusion: CCDC154 knockdown suppressed LC development through reducing Snail expression.

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CCDC154基因敲低通过抑制Snail基因的表达抑制肝癌的生长。

目的：探讨含CCDC154 （coil -coil domain containing 154）在肝癌中的作用。本研究的目的是探讨CCDC154在LC中的作用及其潜在机制。方法：应用UALCAN、Human Protein Atlas和Kaplan-Meier绘图仪网站对CCDC154的表达及预后进行分析。Western blotting法检测蛋白表达。用慢病毒沉默LC细胞中CCDC154的表达。采用细胞计数法、集落形成法和流式细胞术观察LC细胞的增殖和凋亡情况。采用抓伤愈合法和Transwell法观察LC细胞的迁移和侵袭情况。结果：CCDC154在LC中高表达，且与肿瘤分级、分期有关。CCDC154高表达与LC患者预后不良相关。CCDC154的沉默抑制了LC细胞的增殖、迁移和侵袭。它还增加了LC细胞的凋亡。敲除CCDC154后，Twist、Vimentin和Snail的表达下调。过表达Snail可减弱CCDC154敲低对LC细胞生长的抑制作用。结论：CCDC154基因敲低可通过降低Snail基因表达抑制LC的发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Medical Research 医学-医学：研究与实验

CiteScore

3.20

自引率

0.00%

发文量

247

审稿时长

>12 weeks

期刊介绍： European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.