Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector

Abstract

Rice is a staple food in East and Southeast Asian countries, rice disease surveillance and warning is particularly important. Rice stripe disease and southern rice black-streaked dwarf virus disease are the insect-borne viral diseases that easily break out and quickly become epidemic. It is important to improve the efficiency of the virus detection method. In this work, a colloidal gold strip assay (CGSA) was used in mixed samples to detect rice stripe virus (RSV) and southern rice black-streaked dwarf virus (SRBSDV) in small brown planthoppers (SBPH) and white-backed planthoppers (WBPH), respectively. The results show that RSV could be detected in 9, 19 and 49 SBPHs mixed with a single positive SBPH sample, and SRBSDV could be detected in 9 WBPHs mixed with a single positive WBPH sample. The mixed sample detection technology presented in this article can significantly reduce the cost of detection and fulfil the requirements for large-scale, real-time virus surveillance in sentinel surveillance.