Cost-effective, user-friendly detection and preconcentration of thrombin on a sustainable paper-based electrochemical platform

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS Analytical and Bioanalytical Chemistry Pub Date : 2025-02-01 DOI:10.1007/s00216-025-05764-9

Ada Raucci, Giuseppina Sorrentino, Sima Singh, Nicola Borbone, Giorgia Oliviero, Gennaro Piccialli, Monica Terracciano, Stefano Cinti

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Abstract

Thrombin overexpression in serum serves as a critical biomarker and is implicated in several diseases associated with significant morbidity and mortality. Existing techniques for thrombin detection are time-consuming and require sophisticated equipment and extensive sample preparation procedures, which further delay the detection and increase the cost of the procedure. Early and accessible diagnosis at the point of care, especially in limited-resource countries, represents the first step of clinical interventions. To overcome these limitations, we have proposed an innovative, sustainable paper-based electrochemical detection platform for thrombin. In this work, a sustainable paper-based aptasensor was rationally designed, characterized, evaluated against conventional gold standard plastic-based substrates, and applied to human serum, yielding a detection limit of ~ 60 pM. The present method provides an efficient and user-friendly way for the detection of thrombin and potentially leading to better management and treatment outcomes for patients.

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具有成本效益，用户友好的检测和凝血酶在可持续纸基电化学平台上的预浓缩。

凝血酶在血清中的过度表达是一种重要的生物标志物，与几种与显著发病率和死亡率相关的疾病有关。现有的凝血酶检测技术耗时长，需要复杂的设备和大量的样品制备程序，这进一步延迟了检测并增加了程序成本。特别是在资源有限的国家，在护理点提供早期和可获得的诊断是临床干预的第一步。为了克服这些限制，我们提出了一种创新的、可持续的基于纸张的凝血酶电化学检测平台。在这项工作中，合理设计了一种可持续的纸基适体传感器，对其进行了表征，并与传统的金标准塑料基底物进行了评估，并将其应用于人类血清，检测限为~ 60 pM。本方法为凝血酶的检测提供了一种高效且用户友好的方法，并有可能为患者带来更好的管理和治疗结果。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.