Cutin formation in tomato is controlled by a multipartite module of synergistic and antagonistic transcription factors.

IF 6.9 1区生物学 Q1 CELL BIOLOGY Cell reports Pub Date : 2025-02-25 Epub Date: 2025-01-31 DOI:10.1016/j.celrep.2025.115258

Yuan Shi, Changhao Deng, Xiangyin Lu, Yan Wang, Yaowen Pan, Deding Su, Wang Lu, Yuxiang Lin, Rui Li, Junnan Han, Yanwei Hao, Yi Chen, Ghassen Abid, Julien Pirrello, Mondher Bouzayen, Yudong Liu, Zhengguo Li, Baowen Huang

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Abstract

Cuticles protect plants from water loss and pathogen attack. We address here the functional significance of SlGRAS9, SlZHD17, and SlMBP3 in regulating cutin formation in tomato fruit. The study unveils the role of the multipartite "SlGRAS9-SlZHD17-SlMBP3-SlMIXTA-like" transcription factor module in cutin biosynthesis. Plants deficient in SlGRAS9, SlZHD17, or SlMBP3 exhibit thickened cuticles and a higher accumulation of cutin monomers, conferring extended fruit shelf life and higher tolerance to postharvest fungal infection. SlGRAS9 regulation of cutin is mediated by SlZHD17, a negative regulator of SlCYP86A69. SlZHD17 acts synergistically with SlMBP3 to repress SlCYP86A69, and its interaction with SlMIXTA-like prevents the binding to the SlCYP86A69 promoter, thereby releasing the repression of cutin biosynthesis. SlZHD17 and SlMBP3 synergistically repress cutin biosynthesis, while SlMIXTA-like and SlCD2 act antagonistically to SlZHD17 and SlMBP3 on this metabolic pathway. The study defines targets for breeding strategies aimed at improving cuticle-associated traits in tomato and potentially other crops.

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番茄角质素的形成是由协同和拮抗转录因子组成的多重模块控制的。

角质层保护植物免受水分流失和病原体的侵袭。我们在此讨论SlGRAS9， SlZHD17和SlMBP3在调节番茄果实角质形成中的功能意义。该研究揭示了多组分“slgras9 - slzhd17 - slmbp3 - slmixta样”转录因子模块在角质素生物合成中的作用。缺乏SlGRAS9、SlZHD17或SlMBP3的植株表现出更厚的角质层和更高的角质层单体积累，从而延长果实的保质期和对采后真菌感染的耐受性。SlGRAS9对角质素的调控是由SlCYP86A69的负调控因子SlZHD17介导的。SlZHD17与SlMBP3协同作用抑制SlCYP86A69，与slmixta -样相互作用阻止其与SlCYP86A69启动子结合，从而释放对角质素生物合成的抑制。SlZHD17和SlMBP3协同抑制角质蛋白的生物合成，而slmixta -样和SlCD2在这一代谢途径上拮抗SlZHD17和SlMBP3。该研究确定了旨在改善番茄和潜在的其他作物角质层相关性状的育种策略的目标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.