The location and genome origin of alien chromatin in wheat founder parent Xiaoyan 6.

IF 4.4 1区 农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2025-02-01 DOI:10.1007/s00122-025-04826-x
Qiaoling Luo, Qi Zheng, Chunyan Tong, Hongwei Jia, Liqin Liu, Mou Yin, Jingzhong Xie, Hongwei Li, Hongxin Wang, Zeyang Chen, Bin Li, Fei He, Zhensheng Li
{"title":"The location and genome origin of alien chromatin in wheat founder parent Xiaoyan 6.","authors":"Qiaoling Luo, Qi Zheng, Chunyan Tong, Hongwei Jia, Liqin Liu, Mou Yin, Jingzhong Xie, Hongwei Li, Hongxin Wang, Zeyang Chen, Bin Li, Fei He, Zhensheng Li","doi":"10.1007/s00122-025-04826-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>The location of alien chromatin in Xiaoyan 6 was identified using mc-GISH analysis, genetic mapping and whole genome re-sequencing, and its possible origin was discussed. As a founder parent, Xiaoyan 6 has played an important role in distant hybridization breeding in China. Although it came from the cross between common wheat and Thinopyrum ponticum (Podp.) Barkworth and D.R. Dewey, the location of its alien chromatin has not been determined using traditional genomic in situ hybridization (GISH). In the present study, chromosome variation in Xiaoyan 6 was discovered by multicolor GISH analysis. Four alien-specific markers were developed by specific-locus amplified fragment sequencing technique. Their amplified sequences were analyzed by basic local alignment search tool with the reference genome sequences of common wheat Chinese Spring (CS) and Th. elongatum, and the whole genome re-sequencing reads of Th. ponticum and CS. Furthermore, the four markers were mapped on three different chromosomes in two RIL populations. By dissecting the mapped reads depth of the whole genome re-sequencing of Xiaoyan 6, we found that the depth of nine chromosome regions was obviously lower than the average. Among these, three regions on 1A, 3A and 7B were demonstrated as the alien introgressions in Xiaoyan 6 by multiple methods. Finally, the genetic transmission of the alien chromatin was analyzed in a set of wheat-Th. ponticum introgression lines. Some stable QTLs for morphological and physiological traits have been mapped near the alien chromatin.</p>","PeriodicalId":22955,"journal":{"name":"Theoretical and Applied Genetics","volume":"138 2","pages":"41"},"PeriodicalIF":4.4000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theoretical and Applied Genetics","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s00122-025-04826-x","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

Abstract

Key message: The location of alien chromatin in Xiaoyan 6 was identified using mc-GISH analysis, genetic mapping and whole genome re-sequencing, and its possible origin was discussed. As a founder parent, Xiaoyan 6 has played an important role in distant hybridization breeding in China. Although it came from the cross between common wheat and Thinopyrum ponticum (Podp.) Barkworth and D.R. Dewey, the location of its alien chromatin has not been determined using traditional genomic in situ hybridization (GISH). In the present study, chromosome variation in Xiaoyan 6 was discovered by multicolor GISH analysis. Four alien-specific markers were developed by specific-locus amplified fragment sequencing technique. Their amplified sequences were analyzed by basic local alignment search tool with the reference genome sequences of common wheat Chinese Spring (CS) and Th. elongatum, and the whole genome re-sequencing reads of Th. ponticum and CS. Furthermore, the four markers were mapped on three different chromosomes in two RIL populations. By dissecting the mapped reads depth of the whole genome re-sequencing of Xiaoyan 6, we found that the depth of nine chromosome regions was obviously lower than the average. Among these, three regions on 1A, 3A and 7B were demonstrated as the alien introgressions in Xiaoyan 6 by multiple methods. Finally, the genetic transmission of the alien chromatin was analyzed in a set of wheat-Th. ponticum introgression lines. Some stable QTLs for morphological and physiological traits have been mapped near the alien chromatin.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
期刊最新文献
Fine mapping of the unique Ur-11 gene conferring broad resistance to the rust pathogen of common bean. Fine mapping of stripe rust resistance gene YrAn1589 in common wheat using Wheat660K SNP array and BSR-Seq. Optimization of sparse phenotyping strategy in multi-environmental trials in maize. The knockout of Gγ subunit HvGS3 by CRISPR/Cas9 gene editing improves the lodging resistance of barley through dwarfing and stem strengthening. Characterization of a stable QTL for quality-related traits and its effects on yields in wheat (Triticum aestivum L.).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1