Hyun Jong Lee, Ji An Kim, Yerin Lee, Saebin Lim, Yoon Hong Chun
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引用次数: 0
Abstract
Purpose: We present a convenient and safe allergen-specific immunotherapy using injectable hyaluronic acid (HA) hydrogel containing house dust mite (HDM) crosslinked via visible-light-induced thiol-ene reaction.
Methods: We developed 2 types of HDM-containing HA hydrogels, namely thiolated HA (SH-HA) + methacrylated HA (MA-HA) (Gel-1) and 4-arm poly (ethylene glycol) (PEG)-SH + MA-HA (Gel-2). The immunotherapeutic effect of the hydrogels was tested using a murine model of allergic rhinitis. Sensitized mice received 3 subcutaneous injections of the HDM extract (subcutaneous immunotherapy [SCIT] group) or phosphate buffer saline (negative and positive control) at 2-day intervals. Mice in the HA hydrogel immunotherapy groups received one subcutaneous injection of each HA hydrogel precursor solution that formed hydrogel by transmitting blue light through the skin. All except the negative control received HDM extract intranasally for 5 days. Nasal symptoms, ear swelling, eosinophil count, antibody levels, and histopathology of the nasal mucosa were analyzed.
Results: All HDM-containing immunotherapy groups exhibited reduced nasal symptoms, ear swelling, and eosinophil count in nasopharyngeal lavage compared to the positive control group. Eosinophils, mast cells, and goblet cells in the nasal mucosa decreased in all treatment groups compared to the positive control group. The serum levels of HDM-specific immunoglobulin G (IgG)1 increased in all treatment groups; however, IgG2a levels increased only in the SCIT and Gel-2 groups. Interleukin (IL)-4, 13, and 17 decreased in all treatment groups compared to those in the positive control group, whereas IL-10 level increased only in the SCIT and Gel-2 groups. SCIT and Gel-2 treatment showed similar capability to induce regulatory T cells.
Conclusions: Injectable HA hydrogel containing HDM reduced allergic symptoms and induced tolerance in a murine model of allergic rhinitis.
期刊介绍：
The journal features cutting-edge original research, brief communications, and state-of-the-art reviews in the specialties of allergy, asthma, and immunology, including clinical and experimental studies and instructive case reports. Contemporary reviews summarize information on topics for researchers and physicians in the fields of allergy and immunology. As of January 2017, AAIR do not accept case reports. However, if it is a clinically important case, authors can submit it in the form of letter to the Editor. Editorials and letters to the Editor explore controversial issues and encourage further discussion among physicians dealing with allergy, immunology, pediatric respirology, and related medical fields. AAIR also features topics in practice and management and recent advances in equipment and techniques for clinicians concerned with clinical manifestations of allergies and pediatric respiratory diseases.
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