Responses of Littorina spp. Intertidal Snails to Thermal Extremes Indicate Countergradient Variation in Fitness

Abstract

Global change models predict not only a steady increase in temperatures but also an increase in the occurrence of hot and cold extremes. Organisms' responses to thermal extremes will depend on species-specific traits and the degree of within-species variation (among populations), with populations from warmer latitudes often predicted to have higher thermal tolerance than populations from colder latitudes. The evolution of population-specific responses, however, can be limited by gene flow that homogenises populations. Here, we investigate this relationship with a study of the survival of Littorina littorea, L. obtusata, and L. saxatilis—marine snails with varying dispersal potential—collected on either side of a known biogeographic break. Snails were laboratory-acclimated for several weeks before undergoing exposures to extreme heat, extreme cold, or ambient conditions, and individual mortality was recorded after each exposure. In line with common predictions, we observed that the degree of population divergence in survival under thermal extremes was negatively related to dispersal potential, and that populations from the colder latitude generally had higher survival of sub-freezing temperatures. Contrary to common predictions, however, we observed greater survival after extreme heat in populations from colder latitudes than in their warmer-latitude counterparts, a pattern known as countergradient variation. This experiment highlights counterintuitive responses to thermal extremes, emphasising that colder-latitude populations could experience population growth under more extreme climates due to higher survival at both hot and sub-freezing thermal extremes.