RETRACTED: Overexpression of Neural Precursor Cell Expressed Developmentally Downregulated 9 (NEDD9) reduces ox-LDL-induced Anoikis in atherosclerotic vascular endothelial cells

IF 2.5 Q2 CARDIAC & CARDIOVASCULAR SYSTEMS IJC Heart and Vasculature Pub Date : 2025-02-01 Epub Date: 2025-01-17 DOI:10.1016/j.ijcha.2025.101609
Xiaowei Zhou , Qinghua Hu , Meihong Yu , Kaixuan Li
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Abstract

This article has been retracted at the request of the Authors and the Editor-in-Chief: please see Elsevier policy on article withdrawal (https://www.elsevier.com/about/policies-and-standards/article-withdrawal).
Post-publication, the authors identified significant errors in the experimental data and statistical analysis methods presented in the study. Upon thorough review, it was discovered that these inaccuracies compromise the integrity of the findings, and therefore, the authors believe it is in the best interest of the scientific community to retract the paper. The authors apologise for any inconvenience this may cause.
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神经前体细胞表达发育下调9 (NEDD9)的过表达可减少ox- ldl诱导的动脉粥样硬化血管内皮细胞的Anoikis。
目的:本研究旨在探讨Anoikis基因在血管内皮细胞损伤中的作用,探索诊断性生物标志物,为潜在的分子机制提供新的见解,为疾病的检测和治疗提供新的视角。方法:利用Anoikis基因集对gene Expression Omnibus (GEO: GSE100927)数据集进行富集分析,鉴定与动脉粥样硬化相关的交叉基因。进一步研究了Anoikis基因在GSE100927中的表达及通路富集情况。采用最小绝对收缩和选择算子(LASSO)方法进行降维建模,获取动脉粥样硬化相关基因并构建Anoikis评分。逆转录定量聚合酶链反应(RT-qPCR)验证了ox- ldl诱导的Bend.3细胞中NEDD9、FOSB和ERCC1的表达。通过细胞计数试剂盒-8 (CCK8)、5-乙基-2'-脱氧尿苷(EdU)和流式细胞术分析NEDD9在ox-LDL和脱落诱导的Anoikis细胞上的过表达或沉默。结果:Anoikis基因分析显示,动脉粥样硬化组与正常组相比,NFIL3、NR4A3、ADAMTS4、NEDD9、STX17-AS1、CSF3表达过低,FOSB、ERCC1表达过高。LASSO回归分析得出Anoikis评分= -9.522e-01 × NFIL3 - 3.410 × NEDD9 + 2.728e-01 × ADAMTS4 + 1.178 × FOSB + 5.896e-15 × ERCC1 + 1.558e+01。与空白组比较,ox-LDL干预组NEDD9、FOSB、ERCC1均低表达。si-NEDD9促进ox-LDL干预的Bend.3细胞中活性氧(ROS)和凋亡水平的增加。转染e- nedd9可提高ox-LDL和脱离诱导的Bend.3细胞的活力,同时降低ROS和凋亡水平。结论:本研究建立了预测内皮细胞损伤的可靠动脉粥样硬化Anoikis模型。在Anoikis基因中,NEDD9的过表达减少ox-LDL和脱落诱导的内皮细胞Anoikis。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
IJC Heart and Vasculature
IJC Heart and Vasculature Medicine-Cardiology and Cardiovascular Medicine
CiteScore
4.90
自引率
10.30%
发文量
216
审稿时长
56 days
期刊介绍: IJC Heart & Vasculature is an online-only, open-access journal dedicated to publishing original articles and reviews (also Editorials and Letters to the Editor) which report on structural and functional cardiovascular pathology, with an emphasis on imaging and disease pathophysiology. Articles must be authentic, educational, clinically relevant, and original in their content and scientific approach. IJC Heart & Vasculature requires the highest standards of scientific integrity in order to promote reliable, reproducible and verifiable research findings. All authors are advised to consult the Principles of Ethical Publishing in the International Journal of Cardiology before submitting a manuscript. Submission of a manuscript to this journal gives the publisher the right to publish that paper if it is accepted. Manuscripts may be edited to improve clarity and expression.
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