{"title":"The denitrification mechanism and microbial responses of oligotrophic aerobic denitrifying bacteria coupled with various sources biochar","authors":"Yiling Di, Rui Huo, Wanying Li, Chenbin Wu, Shilei Zhou","doi":"10.1016/j.jwpe.2025.106983","DOIUrl":null,"url":null,"abstract":"<div><div>Biochar has a wide range of applications for efficient microbial nitrogen removal. The efficiency and mechanism of nitrogen removal and the dynamic microbial response of corn stover, reed, and bamboo biochar mixed aerobic denitrifying bacteria (CS-BIADB, RE-BIADB, and BB-BIADB) were investigated. There were differences in the characterization and derivatization of different biochar materials. Corn stover biochar possesses a higher capacitance and more alkaline initial derivatives (pH = 9.71); the concentration of aromatic protein I in the initial derivatives of reed biochar is relatively high; the initial derivative concentration of bamboo biochar is the highest. The highest nitrate nitrogen (NO<sub>3</sub><sup>−</sup>-N) removal was observed for RE-BIADB at <em>N</em> = 40 mg/L (67.24 ± 2.64 %); and the highest NO<sub>3</sub><sup>−</sup>-N removal was observed for BB-BIADB at <em>N</em> = 10 mg/L (40.57 ± 4.98 %); in the intermittent experiment, CS-BIADB had the highest final NO<sub>3</sub><sup>−</sup>-N removal efficiency (57.79 ± 1.81 %). The rich functional groups and charging/discharging capacitance of the biochar material contributed to its better performance in long-term nitrogen removal. CS-BIADB always has the highest microbial diversity and abundance. At the end of the experiment, the microbial diversity of different BIADBs increased, while their distinctive ness decreased. Correlation analysis indicated that biochar derivative-like protein I played key roles in improving microbial nitrogen removal efficiency. In addition, network analysis results indicated that <em>Azoarcus</em>, <em>Bdellovibrio,</em> and <em>Comamonadaceae</em> were the key genera for NO<sub>3</sub><sup>−</sup>-N removal in the three BIADBs. In summary, this paper reveals the mechanism of biochar to promote NO<sub>3</sub><sup>−</sup>-N removal by aerobic denitrifying bacteria and provides support for the application to microbial denitrification in oligotrophic waters.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"70 ","pages":"Article 106983"},"PeriodicalIF":6.7000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water process engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214714425000558","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/14 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Biochar has a wide range of applications for efficient microbial nitrogen removal. The efficiency and mechanism of nitrogen removal and the dynamic microbial response of corn stover, reed, and bamboo biochar mixed aerobic denitrifying bacteria (CS-BIADB, RE-BIADB, and BB-BIADB) were investigated. There were differences in the characterization and derivatization of different biochar materials. Corn stover biochar possesses a higher capacitance and more alkaline initial derivatives (pH = 9.71); the concentration of aromatic protein I in the initial derivatives of reed biochar is relatively high; the initial derivative concentration of bamboo biochar is the highest. The highest nitrate nitrogen (NO3−-N) removal was observed for RE-BIADB at N = 40 mg/L (67.24 ± 2.64 %); and the highest NO3−-N removal was observed for BB-BIADB at N = 10 mg/L (40.57 ± 4.98 %); in the intermittent experiment, CS-BIADB had the highest final NO3−-N removal efficiency (57.79 ± 1.81 %). The rich functional groups and charging/discharging capacitance of the biochar material contributed to its better performance in long-term nitrogen removal. CS-BIADB always has the highest microbial diversity and abundance. At the end of the experiment, the microbial diversity of different BIADBs increased, while their distinctive ness decreased. Correlation analysis indicated that biochar derivative-like protein I played key roles in improving microbial nitrogen removal efficiency. In addition, network analysis results indicated that Azoarcus, Bdellovibrio, and Comamonadaceae were the key genera for NO3−-N removal in the three BIADBs. In summary, this paper reveals the mechanism of biochar to promote NO3−-N removal by aerobic denitrifying bacteria and provides support for the application to microbial denitrification in oligotrophic waters.
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The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies
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