Chemical profile, insecticidal and repellent activities of four underexplored Moroccan essential oils against Sitophilus oryzae

IF 2.7 2区农林科学 Q1 ENTOMOLOGY Journal of Stored Products Research Pub Date : 2025-05-01 Epub Date: 2025-02-01 DOI:10.1016/j.jspr.2025.102552

Mbarek Ouabou , Houssam Annaz , Filippo Maggi , Marta Ferrati , Eleonora Spinozzi , Amal El Amrani

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Abstract

This study aims to evaluate the chemical composition and insecticidal and repellent effects of essential oils (EOs) extracted from four underexplored Moroccan plants, i.e., Deverra denudata, Micromeria macrosiphon, Ruta angustifolia, and Thymus riatarum, against the rice weevil Sitophilus oryzae. The chemical composition of the EOs was determined through gas chromatography-mass spectrometry (GC-MS) analysis. Moreover, the insecticidal effect was assessed through fumigant and contact assays, while the repellent effect was evaluated using the area preference assay. Regarding the chemical composition, α-pinene (42.7%) and β-pinene (40.5%) were the major compounds of D. denudata EO, while bornyl acetate (30.2%), borneol (20.4%), and camphor (12.5%) dominated in M. macrosiphon EO. Regarding R. angustifolia EO, 2-undecanone (90.2%) and 2-decanone (7.5%) were identified as the main constituents. Finally, T. riatarum EO was dominated by p-cymene, carvacrol, and borneol in percentages of 26.8, 25.0, and 13.5%, respectively. Regarding the fumigant toxicity, all EOs displayed strong efficacy after 72 h of exposure with LC₅₀ values comprised between 73.9 and 85 μL/L of air, and D. denudata EO appeared to be the most toxic. Regarding the contact toxicity, all EOs exhibited a strong effect, where R. angustifolia EO was the most toxic after 24 h (LC₅₀ of 0.13 μL/insect), and M. macrosiphon after 72 h (LC₅₀ value of 0.08 μL/insect). On the contrary, T. riatarum EO exhibited the highest percentages of repellence (PR of 92%) at the lowest concentration (0.019 μL/cm²) after 30 min, while R. angustifolia EO after 4 h (PR of 90%) at (0.019 μL/cm²). Finally, for the long-term exposure (24 h), T. riatarum exhibited the highest PR of 86 and 94% at 0.019 μL/cm² and 0.156 μL/cm², respectively. Overall, our study has reported for the first time the insecticidal and repellent effects of EOs from the four Moroccan plants against the rice weevil S. oryzae, underscoring their potential as efficient bio-insecticides for the management of stored product pests.

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四种未开发的摩洛哥精油对米象虫的化学特征及杀虫驱避活性

本研究旨在评价四种摩洛哥植物（Deverra denudata、Micromeria macrosiphon、Ruta angustifolia和thyymus riatarum）精油对水稻象鼻虫米象（Sitophilus oryzae）的化学成分和驱虫效果。通过气相色谱-质谱（GC-MS）分析确定了EOs的化学成分。采用熏蒸法和接触法评价杀虫效果，采用面积偏好法评价驱避效果。从化学成分上看，大虹吸野檀的主要成分为α-蒎烯（42.7%）和β-蒎烯（40.5%），其次为乙酸龙脑酯（30.2%）、冰片（20.4%）和樟脑（12.5%）。其中，2-癸酮（90.2%）和2-癸酮（7.5%）为主要成分。对花香烃、香芹酚和冰片分别以26.8%、25.0%和13.5%的比例占主导地位。在熏蒸剂的毒性方面，熏蒸剂的LC50值在73.9 ~ 85 μL/L之间，熏蒸72 h后均表现出较强的熏蒸效果，其中以白骨草熏蒸剂毒性最大。在接触毒性方面，各菌株均表现出较强的作用，其中红叶田鼠的接触毒性在24 h时最大（LC50为0.13 μL/虫），大虹吸田鼠的接触毒性在72 h时最大（LC50为0.08 μL/虫）。与此相反，在最低浓度（0.019 μL/cm2）下，30 min驱避率最高（PR为92%）；在0.019 μL/cm2下，4 h驱避率最高（PR为90%）。结果表明，长时间暴露（24 h）时，白头到老的PR值最高，分别为0.019 μL/cm2和0.156 μL/cm2，达到86%和94%。总的来说，我们的研究首次报道了来自四种摩洛哥植物的EOs对水稻象鼻虫S. oryzae的杀虫和驱避效果，强调了它们作为有效生物杀虫剂管理储存产品害虫的潜力。

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来源期刊

Journal of Stored Products Research 生物-昆虫学

CiteScore

5.70

自引率

18.50%

发文量

112

审稿时长

45 days

期刊介绍： The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.